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1.14.18.1: tyrosinase

This is an abbreviated version!
For detailed information about tyrosinase, go to the full flat file.

Word Map on EC 1.14.18.1

Reaction

2 L-dopa +

O2
= 2 dopaquinone + 2 H2O

Synonyms

AbPPO1, AbPPO4, AbTYR, aurone synthase, catalase-phenol oxidase, catechol oxidase, catecholase, CATPO, chlorogenic acid oxidase, chlorogenic oxidase, cresolase, cresolase/monophenolase, CsPPO, CZA14Tyr, deoxy-tyrosinase, dihydroxy-L-phenylalanine:oxygen oxidoreductase, Diphenol oxidase, diphenolase, dopa oxidase, EC 1.10.3.1, EC 1.14.17.2, Hc-derived phenoloxidase, Hc-phenoloxidase, HcPO, HdPO, hemocyanin-derived phenoloxidase, jrPPO1, jrTYR, L-DOPA monophenolase, L-DOPA oxidase, L-DOPA:oxygen oxidoreductase, L-tyrosine hydroxylase, MdPPO1, melC2, MelC2 tyrosinase, met-tyrosinase, monophenol dihydroxyphenylalanine:oxygen oxidoreductase, monophenol monooxidase, monophenol monooxygenase, monophenol monoxygenase, monophenol oxidase, monophenol oxygen oxidoreductase, monophenol, 3,4-dihydroxy L-phenylalanine (L-DOPA):oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine:oxygen oxidoreductase, monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, monophenol, L-Dopa: oxidoreductase, monophenol, L-DOPA: oxygen oxidoreductase, monophenol, o-diphenol: oxygen oxidoreductase, monophenol, o-diphenol:O2 oxidoreductase, monophenol, o-diphenol:oxygen oxido-reductase, monophenol, o-diphenol:oxygen oxidoreductase, monophenol, polyphenol oxidase, monophenol: dioxygen oxidoreductases, hydroxylating, monophenolase, monphenol mono-oxygenase, More, mTyr, murine tyrosinase, mushroom tyrosinase, mushroom tyrosine, N-acetyl-6-hydroxytryptophan oxidase, o-diphenol oxidase, o-diphenol oxidoreductase, o-diphenol oxygen oxidoreductase, o-diphenol: O2 oxidoreductase, o-diphenol: oxidoreductase, o-diphenol:O2 oxidoreductase, o-diphenol:oxygen oxidoreductase, o-diphenolase, OCA1, Orf13, oxygen oxidoreductase, phenol oxidase, phenol oxidases, phenolase, phenoloxidase, PO, polyaromatic oxidase, polyphenol oxidase, polyphenol oxidase 3, polyphenol oxidase 4, polyphenol oxidase B, polyphenolase, polyphenoloxidase, PotPPO, PPO, PPO 3, PPO B, PPO1, PPO2, PPO3, pro-PO III, prophenoloxidase III, pyrocatechol oxidase, SPRTyr, ST94, ST94t, tryosinase, tryrosinase, TY, tyr, TYR1, TYR2, tyrA, TyrBm, tyrosinase, tyrosinase 2, tyrosinase 4, tyrosinase diphenolase, tyrosine-dopa oxidase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.1 tyrosinase

pH Stability

pH Stability on EC 1.14.18.1 - tyrosinase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11
-
4°C, 20 h, about 75% loss of activity above
636443
2 - 10
-
PPO exhibits a gradual increase in activity from pH 2.0-4.5, PPO exhibits a gradual decline in activity at pH above 4.5 with a slight recovery at pH 10.0
698434
2.5 - 3.5
-
the enzyme is most unstable at pH 2.5-3.5 retaining less than 10% of original activity after 2 h
712619
3 - 10
3 - 8
-
isozyme PPO III, 90% activity after 18 h at pH 3.0
636356
3 - 9
-
the enzyme is extremely stable between pH 3.0 and 9.0 after 24 h incubation at 4°C retaining at least 85% of its original activity
712053
3.5 - 4.5
-
optimal stability at pH 4.5, less stable above pH 6.0
636382
4 - 7
purified native enzyme, stable at
745110
4.5 - 10
-
enzyme SPRTyr, stable at
745561
5
-
1 h, purified recombinant enzyme, loss of 50% activity
673560
5 - 10
5 - 7
-
purified enzyme, high stability in the pH range
746341
5 - 8
-
the soluble enzyme shows half-lives of about 11 min, 35 min, and 3 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively. The cross-linked enzyme aggregates show half-lives of about 11 min, 35 min, and 1 min at pH 5.0-5.5, pH 6.0, and pH 6.5-8.0, respectively
712534
5.5 - 9
-
most stable at pH 7.0, more than 90% of the initial activity remains after 24 h incubation at pH 7, catalase retains more than 60% of its activity at low (pH 5.5) and high (pH 9) values
684646
6 - 7.5
-
more than 50% activity is retained during storage for 72 h at pH 6.0-7.5
712619
6 - 8
-
enzyme is stable in the pH range of 6.0-8.0
687243
6 - 9.5
-
80% activity after 1 h incubation
636370
6.5
-
4°C, 24 h, unstable
636371
6.5 - 10
-
enzyme CZA14Tyr, stable at
745561
6.5 - 11
4°C, 24 h, less than 10% loss of activity
658626
6.5 - 7.4
-
partially purified enzyme, stable at
674018
7 - 9.5
-
4°C, 24 h, cuticle-bound enzyme is stable
636341
8 - 10
-
more than 80% of maximal activity is maintained between pH 9.0 and pH 10.0, high activity in the pH range of 8.0-10.0
745474
8 - 11
TyrA exhibits optimal monophenol and diphenol oxidase activities under basic conditions (pH above 8.0), when the pH is higher than 10.0 the enzyme starts to lose its activity quickly, it loses nearly 50% of activity after 12 h at pH 11.0. Within the pH range of 4.0-10.0, unlike the endogenous enzyme, the recombinant enzyme shows good stability from neutral to moderately alkaline pH, while it is unstable at acidic conditions.
695821
8.5 - 10
-
4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0
636443
9
purified native enzyme, loss of 70% activity
745110
additional information