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0.00072
-
substrate 3-hydroxytyrosine or hydroxyquinone, leaf enzyme extract
0.00084
-
substrate tyrosine, leaf enzyme extract
0.0012
-
wild-type (grown on citrate), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C
0.0014
-
homogentisate 1,2-dioxygenase (grown on citrate), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C
0.003
Coffea guarini
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.004
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.0042
-
transcription regulator mutant (grown on citrate), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C
0.0047
-
transcription regulator mutant (grown on citrate and L-tyrosine), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C
0.0124
-
homogentisate 1,2-dioxygenase mutant (grown on citrate and ferulic acid), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C, 3.8fold lower activity than wild-type
0.019
-
partially purified enzyme, tyrosine hydroxylation
0.0204
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (LtPPO2: 0.34)
0.02912
-
CE, purification, monophenolase activity
0.0402
-
crude extract, at pH 7.0 and 22°C
0.0466
-
wild-type (grown on citrate and ferulic acid), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C, consumption of 46.6 nmol L-tyrosine/min/mg protein after 2 h
0.05512
-
CE, purification, diphenolase activity
0.063
-
PPO-s soluble, crude extract
0.079
-
PPO-s soluble, supernatant 120000g
0.09
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO3: 1.5 nmol/s mg)
0.096
-
PPO-B membrane bound, 6% Triton-X-114
0.173
-
transcription regulator mutant (grown on citrate and ferulic acid), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C, 3.7fold higher activity than wild-type
0.216
values shown are normalized to the specific activity of each PPO for caffeic acid (LtPPO1: 3.6 nmol/s mg)
0.22
-
PPO-B membrane bound, ammoniun sulfate 25-80%
0.228
-
tyrosine hydroxylation
0.348
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO2: 5.8)
0.375
-
DEAE-sepharose, purification of Pseudomonas putida F6 tyrosinase, monophenolase activity
0.45
-
PPO-s soluble, ammoniun sulfate 25-80%
0.8125
-
DEAE-sepharose, purification, diphenolase activity
1.22
purified recombinant enzyme, substrate L-tyrosine, pH 6.8, 25°C
1.43
-
tyrosine hydroxylase activity
1.78
-
partially purified enzyme, pH 6.5, 28°C, substrate phenol
111.75
-
crude enzyme extract, pH 8.5, 15°C
1301
-
catecholase activity
137
Vibrio tyrosinaticus
-
isoenzyme B
1407
-
purified enzyme, pH 6.5, 30°C
15.07
-
purified enzyme, pH 6.5, 22°C
1638
-
purified enzyme, pH 6.5, 30°C
17.3
-
isoenzyme 2, tyrosine hydroxylation
17.5
-
isoenzyme 1, tyrosine hydroxylation
1700
Sigma mushroom tyrosinase (SMT, crude powder, lot 105k7026) (units/mg of powder), WMT and SMT show different levels of tyrosinase activity per milligram of powder
2.143
-
mono Q, specific activity (units/mg) purification, monophenolase activity
2.34
-
tyrosine hydroxylation
2.625
-
after 65.3fold purification, at pH 7.0 and 22°C
2.964
-
gel filtration, purification, monophenolase activity
20.97
-
partially purified enzyme, pH 6.5, 28°C, substrate catechol
23.8
-
purified enzyme, pH 6.5, 22°C
27
-
L-dopa, detected in dormant saffron corms
2847.22
-
purified enzyme, pH 8.5, 15°C
3.058
-
gel slice, purification, monophenolase activity
3.82
-
partially purified enzyme, pH 6.5, 28°C, substrate pyrogallol
30.2
-
reaction with L-Tyr
3000
-
mushroom tyrosinase 3000/mg
32
-
with 4-methylcatechol, trypsin activation
34
purified enzyme, at 30°C
350.02
purified recombinant wild-type intra-melanosomal domain, pH 7.4, 37°C
4
enzyme from cell lysate, at 30°C
4.08
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO3, 68 nmol/s mg)
4.725
-
mono Q, purification, diphenolase activity
4.818
-
gel filtration, purification, diphenolase activity
412
purification step, Superdex 200
442
-
4-hydroxyanisol hydroxylation
46
Vibrio tyrosinaticus
-
isoenzyme A
47.6
purified enzyme, pH 7.0, temperature not specified in the publication
498
Worthington mushroom tyrosinase (WMT, crude powder, lot 33H6588Q) (units/mg of powder), WMT and SMT show different levels of tyrosinase activity per milligram of powder
5.463
-
gel slice, purification, diphenolase activity
5.94
values shown are normalized to the specific activity (in nmol/s mg) of each PPO for caffeic acid (AcPPO1: 99 nmol/s mg)
52.19
-
purified native enzyme, pH 7.0, 35°C
530
-
purified enzyme, pH 6.5, 30°C
600
-
p-cresol, detected in dormant saffron corms
64.2
-
tyrosine hydroxylation
67.18
-
partially purified enzyme, pH 6.5, 28°C, substrate 4-methylcatechol
68
-
partially purified isoenzyme 3
69.5
-
isoenzyme 2, dopa oxidation
7.2
-
with 4-methylcatechol
72
-
isoenzyme 1, dopa oxidation
746
-
purified enzyme, pH 6.5, 30°C
8300
-
mushroom tyrosinase 8300 U/mg
84.34
-
reaction with L-dopa
8839
-
purified native enzyme, pH 7.0, 25°C
900
-
catechol, detected in dormant saffron corms
0.0004
-
substrate coumaric acid, leaf enzyme extract
0.0004
-
substrate hydroxyquinone, leaf enzyme extract
0.0006
Coffea guarini
-
substrate coumaric acid, leaf enzyme extract
0.0006
-
substrate tyrosine, leaf enzyme extract
0.0006
-
substrate coumaric acid, leaf enzyme extract
0.001
Coffea guarini
-
substrate tyrosine, leaf enzyme extract
0.001
-
wild-type (grown on citrate and L-tyrosine), substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C
0.002
-
substrate 3-hydroxytyrosine, leaf enzyme extract
0.002
-
substrate 3-hydroxytyrosine, leaf enzyme extract
3900
-
-
3900
-
mushroom tyrosinase solution (200 U/ml) added in the mushroom tyrosinase inhibition assay
45
-
pyrogallol, detected in dormant saffron corms
additional information
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-
additional information
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-
additional information
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determination of melanin levels
additional information
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100 U/ml mushroom tryrosinase
additional information
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150 U/ml of tyrosinase
additional information
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2400 U/ml mushroom tyrosinase
additional information
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350 Units/ml tyrosinase activity in extracts from Ganoderma lucidum, in extracts from Antrodia camphorata IC50 is about 1 mg/ml
additional information
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activity of mushroom tyrosinase is studied in three ionic liquids, 1-butyl-3-methylimidazolium hexafluorophosphate (1-butyl-3-methylimidazolium hexafluorophosphate), 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]) and 1-butyl-3-methylimidazolium methylsulfate ([BMIm] [MeSO4]), and is compared to that in chloroform. Enzyme follows in ionic liquids basically the same catalytic mechanism as in water
additional information
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aqueous solution of mushroom tyrosinase (1000 U/ml)
additional information
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assay of tyrosinase inhibitory activity with 1000 U/ml mushroom tyrosinase
additional information
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for the mushroom tyrosinase activity assay, mushroom tyrosinase with 100 U/ml is used
additional information
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for tyrosinase inhibitory activity assay mushroom tyrosinase (1000 U/ml) used
additional information
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mushroom tyrosinase purified enzyme assay with 5000 U/ml
additional information
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mushroom tyrosinase solution 40.0 units/ml
additional information
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the monophenolase activity is 3.35 EU/ml, and the diphenolase activity is 189.3 EU/ml. The specific acitivity after purification is 0.71 EU/mg for cresolase and 40.1 EU/mg for catecholase
additional information
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activity is measured spectrophotometrically at 420 nm, 13530.07 EU/mg of protein, gel filtration chromatography
additional information
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enzyme form A, 480.0 DELTA absorbance 470 nm/min/mg, enzyme form B, 258.6 DELTA absorbance 470 nm/min/mg
additional information
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purification of PPO from broccoli florets. Specific activity of crude 800, ammonium sulfate precipitation 3058, DEAE 20425, G-50 31430, G-100 37953 U/min/mg protein
additional information
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assay optimization
additional information
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1064.0 DELTA absorbance 490 nm/min/mg
additional information
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3314 units, 1 unit is the amount of enzyme causing an increase in absorbance at 490 nm of 0.001/min/ml
additional information
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0.0088 units, 1 unit is defined as the amount of enzyme that causes a change in absorbance of 0.001/min
additional information
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9900 units, 1 unit is defined as the amount of enzyme that causes a change in absorbance of 0.001/min
additional information
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the specific activity of the enzyme in the humoral fluid is 4.98 units/min/mg, one unit is defined as 0.001 optical density change per mg protein
additional information
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the crude extract has a specific activity of 4654.2 units/mg and the 26.6fold purified enzyme has a specific activity of 123809.5 units/mg, one unit is defined as the amount of enzyme that causes an increase of absorbance of 0.001 unit/min for 1 ml of enzyme at 25°C
additional information
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26285 U/mg. An increase in absorbance of 0.001 min-1 is taken as one Unit of enzyme activity. G-100, purification step
additional information
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40192 U/mg, phenyl sepharose, purification profile of Barbados cherry PPO. One Unit of enzyme activity is defined as the amount of enzyme that caused an increase in absorbance of 0.001 min-1
additional information
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8.4 ml O2/min/mg
additional information
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assay methods
additional information
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0.281 units, 1 unit is defined as 0.0024 ml O2/min
additional information
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isoenzyme A: 92.0 units, isoenzyme B: 97 units, 1 unit is defined as absorbance change of 0.001 at 430 nm per min
additional information
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no measurable activity in homogentisate 1,2-dioxygenase mutant (grown on citrate and tyrosine) within the 2 h time frame of the enzyme assay, substrate L-tyrosine (1 mM), air saturated 50 mM phosphate buffer, pH 7.0, 30°C
additional information
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172 U/g dry weight and 215 U/g dry weight in unripe and ripe fruits, respectively
additional information
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analysis of browning parameters
additional information
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PPO is immobilized onto MAC400 and MAC200 at various enzyme activities 50000, 100000, 200000, 300000 U/l, at pH 5-8, and at temperature ranging from 10 to 40°C. The optimum conditions for immobilization of PPO onto MAC400 and MAC200 are temperature 40°C, pH 5 and at PPO activity equal to 300000 U/l
additional information
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the specific activity of the crude enzyme is 264 units/mg protein, the specific activity of the 64fold purified enzyme is16853 units/mg protein using 50 mM catechol as substrate in 50 mM sodium phosphate buffer, pH 7.0, at 25°C, one unit of PPO activity is defined as the change in absorbance of 0.001 per minute per ml of enzyme
additional information
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-
additional information
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4359.0 DELTA absorbance/min x 1000/mg
additional information
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35263 units, 1 unit is defined as absorbance change of 0.001 at 430 nm per min
additional information
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time course of PPO activities in embryo and endosperm of maize seeds treated with boron during and following germination is shown
1.14.18.1 tyrosinase
results (
results (
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