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1.14.17.4: aminocyclopropanecarboxylate oxidase

This is an abbreviated version!
For detailed information about aminocyclopropanecarboxylate oxidase, go to the full flat file.

Word Map on EC 1.14.17.4

Reaction

1-aminocyclopropane-1-carboxylate
+
ascorbate
+
O2
=
Ethene
+
cyanide
+
dehydroascorbate
+
CO2
+ 2 H2O

Synonyms

1-aminocyclopropane-1-carboxylate oxidase, 1-aminocyclopropane-1-carboxylic acid oxidase, 1-aminocyclopropane-1-carboxylic acid oxidase (gene CM-ACO1), 1-aminocyclopropane-1-carboxylic oxidase, 1-aminocyclopropyl-1-carboxylic acid oxidase, ACC oxidase, ACC oxidase 1, ACC oxidase 2, ACC-oxidase, ACCO, ACCO1, ACO, Aco1, Aco2, Aco3, ACO4, ACO6, aminocyclopropane-1-carboxylate oxidase, aminocyclopropane-2-carboxylic oxidase, AsACO, AtACO1, ethylene-forming enzyme, LeACO1, LeACO4, Ls-ACO1, Ls-ACO2, Ls-ACO3, MD-ACO1, MD-ACO2, MD-ACO3, NsACO, oxidase, aminocyclopropanecarboxylate, PgACO, PmACO, PsACO, TR-ACO1p, TR-ACO2p, TR-ACO3p, TR-ACO4p, ZmACO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.17.4 aminocyclopropanecarboxylate oxidase

Purification

Purification on EC 1.14.17.4 - aminocyclopropanecarboxylate oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
180fold to near homogeneity
-
ammonium sulfate precipitation, column chromatography, and gel filtration
-
DEAE Sepharose resin column chromatography
-
glutathione-Sepharose chromatography, anion-exchange chromatography
partial
partial, recombinant from Saccharomyces cerevisiae
recombinant ACO is purified by affinity chromatography using nickel nitrilotriacetic acid resin, furthermore Sephadex G-25 resin and a Mono-Q column are used, in addition ACO is extracted from apple leaf and fruit tissue
recombinant enzyme from Escherichia coli to homogeneity
-
recombinant from E. coli, 9.8fold to near homogeneity
-
recombinant from Escherichia coli
-
recombinant GST-fusion protein
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE) plysS by nickel affinity chromatography, ammonium sulfate precipitation
recombinant isozyme ACO1 from Escherichia coli BL21(DE3)-pLysS, to near homogeneity
-
recombinant wild-type enzyme and mutants from Escherichia coli
recombinant wild-type enzyme and mutants from Escherichia coli BL21(DE3) to near homogeneity
-