1.14.17.3: peptidylglycine monooxygenase

This is an abbreviated version!
For detailed information about peptidylglycine monooxygenase, go to the full flat file.

Reaction

Synonyms

alpha-AE, bifunctional PAM, bifunctional peptidylglycine alpha-amidating monooxygenase, CG3832, hPHMcc, More, PAM, PAM-1, PAM-2, PAM-A, PAM-B, PAM/PHM, peptide alpha-amidating enzyme, peptide alpha-amide synthase, peptide-alpha-amide synthetase, peptidyl alpha-amidating enzyme, peptidyl alpha-hydroxylating monooxygenase, peptidyl-glycine alpha-amidating monooxygenase, peptidylglycine 2-hydroxylase, peptidylglycine alpha-amidating mono-oxygenase, peptidylglycine alpha-amidating monooxygenase, peptidylglycine alpha-hydroxylase, peptidylglycine alpha-hydroxylating monooxygenase, peptidylglycine alpha-hydroxylating-monooxygenase, peptidylglycine alpha-monooxygenase, peptidylglycine monooxygenase, peptidylglycine-alpha-amidating monooxygenase, PHM, PHMcc, synthase, peptide alpha-amide, type A PAM

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.17.3 peptidylglycine monooxygenase

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Results	in table
6	Activating Compound
644	AA Sequence
9	Application
1	CAS Registry Number
43	Cloned(Commentary)
70	Cofactor
2	Crystallization (Commentary)
24	Disease/ Diagnostics
1	Expression
24	General Information
4	General Stability
24	IC50 Value
162	Inhibitors
1	kcat/KM [mM/s]
79	Ki Value [mM]
168	KM Value [mM]
43	Localization
81	Metals/Ions
37	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
101	Organism
3	Oxidation Stability
31	PDB ID
48	pH Optimum
5	pH Range
1	pH Stability
2	Posttranslational Modification
47	Protein Variants
44	Purification (Commentary)
30	Reaction
29	Reaction Type
99	Reference
116	Source Tissue
52	Specific Activity [micromol/min/mg]
372	Substrates and Products (Substrate)
19	Subunits
193	Synonyms
1	Systematic Name
31	Temperature Optimum [°C]
1	Temperature Stability [°C]
30	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

5

(25R)-3beta-hydroxycholest-5-en-27-oate

Rattus norvegicus

-

100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, H2O2

674739

0.02

acetyl-L-Tyr-L-Phe-Gly

Xenopus laevis

-

-

438576

-

51

alpha-hydroxyhippuric acid

Cricetulus griseus

-

0.4 mM ferrocyanide, 100 mM MES pH 6.0, 30 mM KCl, 1 microM CuSO4

672113

0.13

D-Tyr-Val-Gly

Xenopus laevis

-

pH 7.5, 37°C, recombinant enzyme

660417

17

dansyl-Gly-Gly-Ser-CO-NH-CH2-COOH

Rattus norvegicus

-

pH 6.0, 37°C, recombinant enzyme

658976

0.08 - 13.8

dansyl-L-Tyr-L-Val-Gly

4 entries

0.02 - 39.1

hippuric acid

5 entries

37

HOOC-NH-CH2-COOH

Rattus norvegicus

-

pH 6.0, 37°C, recombinant enzyme

658976

0.0035 - 15

N-acetyl-Tyr-Val-Gly

6 entries

3 - 14.5

N-benzoylglycine

2 entries

0.143 - 9.2

N-dansyl-L-Tyr-L-Val-Gly

3 entries

-

0.35 - 5

N-dansyl-Tyr-Val-Gly

2 entries

1.4

N-trinitrophenyl-D-Tyr-Val-Gly

Xenopus laevis

-

pH 6.0, 37°C, recombinant enzyme

660417

additional information

additional information

Rattus norvegicus

-

intrinsic rate constants for wild-type and mutant Y318F enzyme

657976

-

0.08

dansyl-L-Tyr-L-Val-Gly

Homo sapiens

-

mutant enzyme H107A, in100 mM MES pH 5.5, at 37°C

727016

0.11

dansyl-L-Tyr-L-Val-Gly

Homo sapiens

-

mutant enzyme H108A, in100 mM MES pH 5.5, at 37°C

727016

4.6

dansyl-L-Tyr-L-Val-Gly

Homo sapiens

-

mutant enzyme M109I, in100 mM MES pH 5.5, at 37°C

727016

13.8

dansyl-L-Tyr-L-Val-Gly

Homo sapiens

-

wild type enzyme, in100 mM MES pH 5.5, at 37°C

727016

0.02

hippuric acid

Xenopus laevis

-

pH 5.0, 37°C, recombinant enzyme

660417

13.6

hippuric acid

Rattus norvegicus

-

pH 6.0, 37°C, [2H2]-hippuric acid, mutant Y318F

657976

25

hippuric acid

Rattus norvegicus

-

pH 6.0, 37°C, [2H2]-hippuric acid, wild-type enzyme

657976

27.6

hippuric acid

Rattus norvegicus

-

pH 6.0, 37°C, [1H2]-hippuric acid, mutant Y318F

657976

39.1

hippuric acid

Rattus norvegicus

-

pH 6.0, 37°C, [1H2]-hippuric acid, wild-type enzyme

657976

0.0035

N-acetyl-Tyr-Val-Gly

Rattus norvegicus

-

purified recombinant mutant Y79W, pH 7.0

658004

0.09

N-acetyl-Tyr-Val-Gly

Rattus norvegicus

-

purified recombinant mutant Y79W, pH 5.5

658004

0.53

N-acetyl-Tyr-Val-Gly

Rattus norvegicus

-

purified recombinant wild-type enzyme, pH 7.0

658004

0.62

N-acetyl-Tyr-Val-Gly

Rattus norvegicus

-

purified recombinant mutant Q170A, pH 7.0

658004

14

N-acetyl-Tyr-Val-Gly

Rattus norvegicus

-

purified recombinant mutant Q170A, pH 5.5

658004

15

N-acetyl-Tyr-Val-Gly

Rattus norvegicus

-

purified recombinant wild-type enzyme, pH 5.5

658004

3

N-benzoylglycine

Rattus norvegicus

-

15°C

438596

14.5

N-benzoylglycine

Rattus norvegicus

-

37°C

438596

0.143

N-dansyl-L-Tyr-L-Val-Gly

Bos taurus

-

-

438575

-

0.983

N-dansyl-L-Tyr-L-Val-Gly

Sus scrofa

-

-

438577

-

9.2

N-dansyl-L-Tyr-L-Val-Gly

Rattus norvegicus

-

100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, ascorbate

674739

-

0.35

N-dansyl-Tyr-Val-Gly

Homo sapiens

P19021

pH 6.5, 37°C

720977

5

N-dansyl-Tyr-Val-Gly

Rattus norvegicus

-

-

438574