1.14.16.7: phenylalanine 3-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 3-monooxygenase, go to the full flat file.

Reaction

a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

PacX, phenylalanine 3-hydroxylase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor

1.14.16.7 phenylalanine 3-monooxygenase

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Results	in table
1	Activating Compound
2	AA Sequence
1	Cloned(Commentary)
1	General Information
2	KM Value [mM]
1	Metals/Ions
1	Natural Substrates/ Products (Substrates)
1	Organism
1	pH Optimum
3	Protein Variants
1	Purification (Commentary)
1	Reaction
1	Reference
3	Substrates and Products (Substrate)
2	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 1.14.16.7 - phenylalanine 3-monooxygenase

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C187F

Streptomyces coeruleorubidus

F5BFC8

hydroxylation rate is reduced to less than 4% compared to the rate of wild-type enzyme

724321

C187F/T202G

Streptomyces coeruleorubidus

F5BFC8

hydroxylation rate is comparable to that of wild-type enzyme. Less than 8% of m-Tyr compared to p-Tyr is formed

724321

T202G

Streptomyces coeruleorubidus

F5BFC8

hydroxylation rate is comparable to that of wild-type enzyme. The ratio of product p-Tyr to m-Tyr is changed to 2:1 for the T202G mutant, which represents a 60-fold increase in the preference for p-Tyr over m-Tyr formation

724321