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1.14.16.5: alkylglycerol monooxygenase

This is an abbreviated version!
For detailed information about alkylglycerol monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.5

Reaction

1-O-alkyl-sn-glycerol
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
1-O-(1-hydroxyalkyl)-sn-glycerol
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

AGMO, alkylglycerol mono-oxygenase, alkylglycerol monooxygenase, EC 1.14.99.17, glyceryl ether hydroxylase, glyceryl ether monooxygenase, glyceryl ether oxidase, glyceryl ether oxygenase, glyceryl ether-cleaving enzyme, glyceryl etherase, glyceryl-ether cleaving enzyme, O-alkylglycerol monooxygenase, oxygenase, glyceryl ether mono-, PRUB680

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.5 alkylglycerol monooxygenase

Engineering

Engineering on EC 1.14.16.5 - alkylglycerol monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C231A
-
the mutant enzyme activity is not significantly different from wild type
C397A
-
the mutant enzyme activity is not significantly different from wild type
D153A
-
the mutant enzyme activity is not significantly different from wild type
D384A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
E137A
-
the mutation leads to an 18fold increase in the Michaelis-Menten constant for tetrahydrobiopterin. The mutant has an approximately 5fold lower affinity for iron
E152A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
E203A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
E212A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
E324K
the mutation is associated with an autosomal recessive microcephaly
H189A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
H220A
-
the mutant enzyme activity is not significantly different from wild type
K234R
-
the mutation is associated with relapses in visceral leishmaniasis
Q146A
-
the mutant enzyme activity is not significantly different from wild type
Q166A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
Q191A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
Q197A
-
the mutant has activity of less than 5% of that of the wild type
R396A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
R405X
-
the mutation is associated with relapses in visceral leishmaniasis
Y174A
-
the mutant has significantly reduced, but clearly detectable, enzyme activity
Y230A
-
the mutant enzyme activity is not significantly different from wild type
Y338A
-
the mutant has activity of less than 5% of that of the wild type
D233A
-
inactive
Q197A
-
the mutant retains minimal activity
additional information
-
mutation of each of 8 conserved His residues (to alanine) which are all essential for activity