1.14.16.2: tyrosine 3-monooxygenase
This is an abbreviated version!
For detailed information about tyrosine 3-monooxygenase, go to the full flat file.
Word Map on EC 1.14.16.2
-
1.14.16.2
-
dopamine
-
dopaminergic
-
parkinsonism
-
nigra
-
striatum
-
catecholamine
-
substantia
-
nerve
-
ventral
-
fiber
-
sympathetic
-
innervation
-
adrenal
-
noradrenergic
-
neurotransmitter
-
6-hydroxydopamine
-
ganglia
-
th-positive
-
catecholaminergic
-
neuropeptide
-
norepinephrine
-
nigrostriatal
-
midbrain
-
monoamine
-
neurotrophic
-
compacta
-
coeruleus
-
hypothalamus
-
tegmental
-
mesencephalic
-
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
-
l-dopa
-
6-ohda
-
neurochemical
-
accumbens
-
noradrenaline
-
rostrally
-
dopac
-
apomorphine
-
perikarya
-
intrastriatal
-
beta-hydroxylase
-
mptp-induced
-
varicosity
-
ventrolateral
-
dopamine-beta-hydroxylase
-
preoptic
-
npy
-
phenylethanolamine
-
analysis
-
diagnostics
-
homovanillic
-
synthesis
- 1.14.16.2
- dopamine
-
dopaminergic
- parkinsonism
- nigra
- striatum
- catecholamine
-
substantia
- nerve
-
ventral
- fiber
-
sympathetic
-
innervation
- adrenal
-
noradrenergic
-
neurotransmitter
- 6-hydroxydopamine
- ganglia
-
th-positive
-
catecholaminergic
-
neuropeptide
- norepinephrine
-
nigrostriatal
- midbrain
-
monoamine
-
neurotrophic
- compacta
- coeruleus
- hypothalamus
- tegmental
-
mesencephalic
- 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
- l-dopa
-
6-ohda
-
neurochemical
- accumbens
- noradrenaline
-
rostrally
-
dopac
- apomorphine
- perikarya
-
intrastriatal
- beta-hydroxylase
-
mptp-induced
-
varicosity
-
ventrolateral
- dopamine-beta-hydroxylase
-
preoptic
- npy
- phenylethanolamine
- analysis
- diagnostics
-
homovanillic
- synthesis
Reaction
Synonyms
CAT-2, DTH1, DTH2, hTH2, L-tyrosine hydroxylase, monophenol monooxygenase, oxygenase, tyrosine 3-mono-, TH, TH1, TH2, TyrH, tyrosinase, tyrosine 3-hydroxylase, tyrosine 3-monooxygenase, tyrosine hydroxylase, tyrosine hydroxylase type 1, tyrosine-3-mono-oxygenase, tyrosine-3-monooxygenase
ECTree
Advanced search results
KM Value
KM Value on EC 1.14.16.2 - tyrosine 3-monooxygenase
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0.34
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydropteridine
-
30°C
0.023
-
pH 7.0, 30°C, mutant enzyme S19E/S40E
0.028
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S8E
0.032
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S19A
0.033
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S19A
0.033
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S19E
0.034
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, wild-type enzyme
0.035
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S40A
0.035
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S40E
0.035
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S8E
0.036
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S8A
0.037
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S19E
0.037
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S19E/S40E
0.037
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S40E
0.039
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S8A
0.04
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, wild-type enzyme
0.045
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S40A
0.047
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S31E
0.048
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S31A
0.051
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S31A
0.051
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
pH 7.0, 30°C, mutant enzyme S31E
0.0001
-
telencephalon extract, 10 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00015
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
telencephalon extract, 1 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.0002
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
hypothalamus extract, 10 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.0002
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
telencephalon extract, 1 nM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00022
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
hypothalamus extract, 1 nM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00024
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
hypothalamus extract, 1 microM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00024
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
telencephalon extract, 1 fM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00026
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
hypothalamus extract, 1 fM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00034
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
hypothalamus extract, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00035
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
telencephalon extract, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00045
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
hypothalamus extract, 0.01 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00048
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
telencephalon extract, 0.01 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00054
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
telencephalon extract, 1 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.00058
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
hypothalamus extract, 1 mM estadriol-17beta, 0.1-0.5 mM L-tyrosine, 1-8 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, PBS buffer pH 6.2, 2-mercaptoethanol, catalase, 30°C
0.045
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine
-
0.1 mM L-tyrosine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C
0.033
recombinant wild-type, pH 7.1
0.055
6-Methyl-5,6,7,8-tetrahydropterin
recombinant wild-type, pH 6.0
0.059
6-Methyl-5,6,7,8-tetrahydropterin
recombinant mutant Y371F, pH 7.1
0.065
6-Methyl-5,6,7,8-tetrahydropterin
recombinant mutant Y371F, pH 6.0
0.146
L-Dopa
-
recombinant, dopa-oxidase activity with 6,7-dimethyltetrahydropterin
0.0016
mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C
0.0053
L-phenylalanine
mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C
0.0092
L-phenylalanine
mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C
0.0105
L-phenylalanine
mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C
0.0144
L-phenylalanine
mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C
0.0148
L-phenylalanine
mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C
0.0152
L-phenylalanine
mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C
0.0164
L-phenylalanine
mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C
0.019
L-phenylalanine
mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C
0.021
L-phenylalanine
mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C
0.022
L-phenylalanine
mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C
0.028
L-phenylalanine
mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C
0.045
L-phenylalanine
mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C
0.0565
L-phenylalanine
mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C
0.062
L-phenylalanine
mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C
0.1
L-phenylalanine
wild type enzyme, in 50 mM HEPES (pH 7.0), at 30°C
0.2
L-phenylalanine
mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C
5
L-phenylalanine
mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C
0.00105
mutant enzyme D425T, in 50 mM HEPES (pH 7.0), at 30°C
0.005
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.3 mM tetrahydropterin
0.006
L-tyrosine
mutant enzyme D425L, in 50 mM HEPES (pH 7.0), at 30°C
0.0069
L-tyrosine
mutant enzyme D425M, in 50 mM HEPES (pH 7.0), at 30°C
0.008
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.3 mM tetrahydropterin
0.0081
L-tyrosine
mutant enzyme R37E/R38E, pH and temperature not specified in the publication
0.0089
L-tyrosine
mutant enzyme D425S, in 50 mM HEPES (pH 7.0), at 30°C
0.009
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.3 mM tetrahydropterin
0.009
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.3 mM tetrahydropterin
0.009
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.3 mM tetrahydropterin
0.009
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.3 mM tetrahydropterin
0.009
L-tyrosine
-
pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.3 mM tetrahydropterin
0.01
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.3 mM tetrahydropterin
0.0106
L-tyrosine
mutant enzyme D425G, in 50 mM HEPES (pH 7.0), at 30°C
0.011
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.3 mM tetrahydropterin
0.011
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.3 mM tetrahydropterin
0.014
L-tyrosine
mutant enzyme K366L, pH and temperature not specified in the publication
0.015
L-tyrosine
mutant enzyme D425C, in 50 mM HEPES (pH 7.0), at 30°C
0.015
L-tyrosine
mutant enzyme E362R/E365R, pH and temperature not specified in the publication
0.016
L-tyrosine
wild type enzyme, pH and temperature not specified in the publication
0.016
L-tyrosine
mutant enzyme D361N, pH and temperature not specified in the publication
0.016
L-tyrosine
mutant enzyme E362G, pH and temperature not specified in the publication
0.017
L-tyrosine
-
0-0.4 mM L-tyrosine, 1 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 200 mM Na-HEPES pH 7.0, 100 mM 2-mercaptoethanol, 0.2 mg/ml catalase, 37°C
0.0172
L-tyrosine
mutant enzyme D425F, in 50 mM HEPES (pH 7.0), at 30°C
0.0187
L-tyrosine
mutant enzyme D425H, in 50 mM HEPES (pH 7.0), at 30°C
0.019
L-tyrosine
mutant enzyme E365G, pH and temperature not specified in the publication
0.02
L-tyrosine
mutant enzyme A297L, pH and temperature not specified in the publication
0.025
L-tyrosine
mutant enzyme S368A, pH and temperature not specified in the publication
0.026
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.03
L-tyrosine
mutant enzyme Y423A, in 50 mM HEPES (pH 7.0), at 30°C
0.0308
L-tyrosine
mutant enzyme D425Y, in 50 mM HEPES (pH 7.0), at 30°C
0.035
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.04
L-tyrosine
mutant enzyme D425V, in 50 mM HEPES (pH 7.0), at 30°C
0.041
L-tyrosine
-
pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.041
L-tyrosine
mutant enzyme D425I, in 50 mM HEPES (pH 7.0), at 30°C
0.042
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.044
L-tyrosine
mutant enzyme Q424A, in 50 mM HEPES (pH 7.0), at 30°C
0.046
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.047
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.047
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.051
L-tyrosine
mutant enzyme T427A, in 50 mM HEPES (pH 7.0), at 30°C
0.053
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.055
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.4 mM 6-0.053 methyltetrahydropterin
0.056
L-tyrosine
mutant enzyme D425A, in 50 mM HEPES (pH 7.0), at 30°C
0.059
L-tyrosine
-
pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin
0.066
L-tyrosine
mutant enzyme Q426A, in 50 mM HEPES (pH 7.0), at 30°C
0.08
L-tyrosine
-
0.38 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C
0.088
L-tyrosine
mutant enzyme D425R, in 50 mM HEPES (pH 7.0), at 30°C
0.097
L-tyrosine
mutant enzyme D425E, in 50 mM HEPES (pH 7.0), at 30°C
0.162
L-tyrosine
mutant enzyme D425Q, in 50 mM HEPES (pH 7.0), at 30°C
0.172
L-tyrosine
mutant enzyme D425N, in 50 mM HEPES (pH 7.0), at 30°C
2.39
L-tyrosine
mutant enzyme D425K, in 50 mM HEPES (pH 7.0), at 30°C
0.0056
tetrahydrobiopterin
wild type, phosphorylated protein, pH 7.2, 30°C
0.0068
tetrahydrobiopterin
wild type protein, dopamine bound but removed from low-affinity site, pH 7.2, 30°C
0.0072
tetrahydrobiopterin
E332D phosphorylated mutant protein, pH 7.2, 30°C
0.0099
tetrahydrobiopterin
L294Y phosphorylated mutant protein, pH 7.2, 30°C
0.012
tetrahydrobiopterin
F300Y phosphorylated mutant protein, pH 7.2, 30°C
0.017
tetrahydrobiopterin
A297L phosphorylated mutant protein, pH 7.2, 30°C
0.027
tetrahydrobiopterin
A297L mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C
0.027
tetrahydrobiopterin
Y371F mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C
0.029
tetrahydrobiopterin
L294A phosphorylated mutant protein, pH 7.2, 30°C
0.029
tetrahydrobiopterin
Y371F phosphorylated mutant protein, pH 7.2, 30°C
0.034
tetrahydrobiopterin
E332D mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C
0.055
tetrahydrobiopterin
-
W166F/W233F/W372F/F14W mutant protein, pH 7, 25°C
0.065
tetrahydrobiopterin
-
W166F/W233F/W372F/F74W mutant protein, pH 7, 25°C
0.07
tetrahydrobiopterin
F300A phosphorylated mutant protein, pH 7.2, 30°C
0.077
tetrahydrobiopterin
-
W166F/W233F/W372F/F34W mutant protein, pH 7, 25°C
0.134
tetrahydrobiopterin
wild type protein, dopamine bound to the high-affinity site, pH 7.2, 30°C
0.356
tetrahydrobiopterin
wild type protein, in the presence of dopamine, pH 7.2, 30°C
0.507
tetrahydrobiopterin
wild type, phosphorylated protein, in the presence of dopamine, pH 7.2, 30°C
0.01
-
recombinant phosphorylated hTH1, L-dopa-oxidase activity
additional information
additional information
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Km-values of diverse tetrahydropterin analogues, substituted at C6, C7 or C3
-
additional information
additional information
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phosphorylation converts the enzyme from a form possessing a high Km for pterin cofactor to a form with a low Km for pterin cofactor
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additional information
additional information
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rat brain: 2 kinetically distinguishable forms: low Km form, high Km form
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additional information
additional information
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rat brain: 2 kinetically distinguishable forms: low Km form, high Km form
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additional information
additional information
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Km is dependent on substrate and cofactor concentration when (6R)-L-erythro-tetrahydrobiopterin is used due to inhibitory effects
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additional information
additional information
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Km dependence on substrate concentrations
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additional information
additional information
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Km dependence on substrate concentrations
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additional information
additional information
Km-values for tyrosine of mutant phenylalanine hydroxylase with tyrosine hydroxylation activity
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additional information
additional information
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Km dependent on cosubstrate type and concentration, overview for 4 isoforms
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additional information
additional information
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both the cofactor (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin and the feedback inhibitor dopamine increase the kinetic stability of human isozyme 1 in vitro
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additional information
additional information
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kinetics analysis, overview
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