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1.14.16.1: phenylalanine 4-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.1

Reaction

L-phenylalanine
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
L-tyrosine
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

cePAH, DicPAH, EC 1.14.3.1, EC 1.99.1.2, HPAH, L-phenylalanine 4-hydroxylase, oxygenase, phenylalanine 4-mono-, P4H, PAH, PheH, phenylalaninase, phenylalanine 4-hydroxylase, phenylalanine hydroxylase, phenylalanine monooxygenase, PheOH, phhA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.1 phenylalanine 4-monooxygenase

Cloned

Cloned on EC 1.14.16.1 - phenylalanine 4-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DicPAH (residue 1-415) is expressed in Escherichia coli
-
DNA and amino acid sequence analysis of wild-type and mutant enzymes, expression of His6-tagged, catalytically active, heterozygous enzymes with one mutated allele
DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, overview
DNA and amino acid sequence determination and analysis, genotyping, expression of mutant enzymes in HEK-193 cells
expressed in COS-7 cells
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli as a recombinant protein
-
expressed in Escherichia coli as fusion proteins with maltose-binding protein
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)/pLysS cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BLR-DE3 cells
expressed in Escherichia coli BW25113DELTAtnaA cells
expressed in Escherichia coli TB1 cells
expressed in Hep-G2 cells
expressed in Lactobacillus plantarum strain CM PUJ411
expression as maltose-binding-protein fusion protein in Escherichia coli circumvents proteolytic degradation by the host cell
-
expression in Escherichia coli
expression of catalytic core mutant DELTA 117PheH and point mutants thereof in Escherichia coli strain C41(DE3)
-
expression of double truncated mutant enzyme DELTAN1-102/DELTAC428-452 in Escherichia coli
expression of His-tagged wild-type, I65T, R261Q and V388M mutant enzymes in Escherichia coli
-
expression of truncated enzyme containing the catalytic domain and various mutants in Escherichia coli
-
expression of wild-type and S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme in Escherichia coli
-
expression of wild-type and T427P mutant enzyme in Escherichia coli
-
expression of wild-type enzyme and Asp112-Lys452, Ser2-Gln428 and Gly103-Gln428 deletion mutants in Escherichia coli
-
expression of wild-type, L348V, L349L and V388M mutant enzyme maltose-binding-protein fusions in Escherichia coli and COS cells
expression of wild-type, R270K and V388M mutant enzymes in the presence of the chemical chaperone glycerol
gene pah, DNA and amino acid sequence determination and analysis, genotyping of a Southern Italian population, overview
-
gene pah, DNA and amino acid sequence determination and analysis, genotyping, overview
-
gene pah, DNA and amino acid sequence determination of wild-type and mutant enzymes, genotyping
-
gene pah, expression in Escherichia coli
gene pah, expression of His-tagged enzyme in fusion with HIV transactivator of transcription protein in Escherichia coli strain BL21(DE3), subcloning in Escherichi acoli strain DH5alpha
-
gene pah, expression of His6-tagged wild-type and mutant enzymes in Escherichia coli
gene pah, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene pah-1, expression as maltose-binding protein fusion protein in Escherichia coli
Q9XYQ5
gene PHA, DNA sequence determination, structural and functional analyses of mutations of the PHA gene, expression of wild-type and mutant enzymes in COS-7 cells, overview
-
identification and sequence analysis of enzyme mutant genes with exon deletions isolated from 59 czech phenylketonuria patients, multiplex ligation-dependent probe amplification method
-
mutant enzymes are expressed in Escherichia coli
regulatory domain (amino acids 1-118) of rat phenylalanine hydroxylase is expressed in Escherichia coli
-
the catalytic domain is expressed in Escherichia coli BL21(DE3) cells
the truncated enzyme PheHDELTA117 is expressed in Escherichia coli BL21(DE3) cells
-
truncated enzyme containing the C-terminal 336 amino acids bearing the catalytic domain
-
wild type and mutant enzyme S231F are expressed in Escherichia coli BL21(DE3) and human hepatoma cells, in the cells supplemented with sepiapterin the amount of expressed S231F PAH increases up to 25%