1.14.15.18: calcidiol 1-monooxygenase

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For detailed information about calcidiol 1-monooxygenase, go to the full flat file.

Reaction

Synonyms

1-hydroxylase-25-hydroxyvitamin D3, 1-OHase, 1alpha,25-dihydroxyvitamin D hydroxylase, 1alpha-hydroxylase, 1alpha-OHase, 1alphaOHase, 25 hydroxy-vitamin D3-1alpha hydroxylase, 25(OH)VD3 1alpha-hydroxylase, 25-hydroxy D3-1alpha-hydroxylase, 25-hydroxycholecalciferol 1-hydroxylase, 25-hydroxycholecalciferol 1-monooxygenase, 25-hydroxycholecalciferol 1alpha-hydroxylase, 25-hydroxycholecalciferol-1-hydroxylase, 25-hydroxyvitamin D 1alpha-hydroxylase, 25-hydroxyvitamin D-1 alpha-hydroxylase, 25-hydroxyvitamin D-1-alpha-hydroxylase, 25-hydroxyvitamin D-1alpha-hydroxylase, 25-hydroxyvitamin D3 1alpha-hydroxylase, 25-hydroxyvitamin D3-1alpha-hydroxylase, 25-hydroxyvitamin-D3-1alpha-hydroxylase, CYB27B1, CYP1alpha, CYP27A, CYP27B1, cytochrome P450 25-hydroxyvitamin D3-1alpha-hydroxylase, EC 1.14.13.13, extra-renal 25-hydroxyvitamin D-1alpha-hydroxylase, extra-renal 25-hydroxyvitamin D3 1alpha-hydroxylase, hydroxylase, 25-hydroxcholecalciferol 1-, More, oxygenase, 25-hydroxycholecalciferol 1-mono-, P450 cytochrome 25-hydroxyvitamin D3 1alpha-hydroxylase, VD3 25-hydroxylase, VDH, vitamin D 1alpha-hydroxylase, vitamin D hydroxylase, vitamin D3 25- and 1alpha-hydroxylase, vitamin D3 25-hydroxylase, vitamin D3 hydroxylase, [25(OH)D3]-1alpha-hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.15.18 calcidiol 1-monooxygenase

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Results	in table
27	Activating Compound
13	AA Sequence
3	Application
1	CAS Registry Number
15	Cloned(Commentary)
45	Cofactor
2	Crystallization (Commentary)
1665	Disease/ Diagnostics
9	Expression
3	General Information
1	General Stability
74	Inhibitors
6	kcat/KM [mM/s]
4	Ki Value [mM]
29	KM Value [mM]
32	Localization
5	Metals/Ions
17	Molecular Weight [Da]
54	Natural Substrates/ Products (Substrates)
85	Organism
5	Pathway
6	pH Optimum
1	pH Range
1	Posttranslational Modification
18	Protein Variants
12	Purification (Commentary)
31	Reaction
3	Reaction Type
85	Reference
140	Source Tissue
31	Specific Activity [micromol/min/mg]
4	Storage Stability
137	Substrates and Products (Substrate)
5	Subunits
90	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
14	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.14.15.18 - calcidiol 1-monooxygenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Pseudonocardia autotrophica

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695379

purified recombinant wild-type Vdh and mutant Vdh-K1 in complex with substrates VD3 and 25(OH)VD3, 20 mg/ml wild-type protein in 20 mM Tris-HCl, pH 7.5, supplemented with 0-0.2% PMbetaCD for VD3 complex or 0-0.2% gammaCD for 25(OH)VD3 complex, 20 mg/ml mutant protein in 20 mM Tris-HCl, pH 7.5, supplemented with 20 mM NaCl, sitting-drop method, 20°C. Vdh-WT crystals are grown using reservoir solution containing 0.1 M BisTris, pH 7.5, 50 mM CaCl2, 40-120 mM NaCl or KCl, and 32-40% PEG 400 or PEG 550 monomethyl ether or 20% PEG 1000, Vdh-K1 is crystallized using reservoir solution containing 0.1 M calcium acetate and 10-14% PEG 3350, X-ray diffraction structure determination and analysis at 1.75-3.05 A resolution

Pseudonocardia autotrophica

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712472