Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.15.18: calcidiol 1-monooxygenase

This is an abbreviated version!
For detailed information about calcidiol 1-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.18

Reaction

calcidiol
+ 2 reduced adrenodoxin + 2 H+ +
O2
=
calcitriol
 + 2 oxidized adrenodoxin +
H2O

Synonyms

1-hydroxylase-25-hydroxyvitamin D3, 1-OHase, 1alpha,25-dihydroxyvitamin D hydroxylase, 1alpha-hydroxylase, 1alpha-OHase, 1alphaOHase, 25 hydroxy-vitamin D3-1alpha hydroxylase, 25(OH)VD3 1alpha-hydroxylase, 25-hydroxy D3-1alpha-hydroxylase, 25-hydroxycholecalciferol 1-hydroxylase, 25-hydroxycholecalciferol 1-monooxygenase, 25-hydroxycholecalciferol 1alpha-hydroxylase, 25-hydroxycholecalciferol-1-hydroxylase, 25-hydroxyvitamin D 1alpha-hydroxylase, 25-hydroxyvitamin D-1 alpha-hydroxylase, 25-hydroxyvitamin D-1-alpha-hydroxylase, 25-hydroxyvitamin D-1alpha-hydroxylase, 25-hydroxyvitamin D3 1alpha-hydroxylase, 25-hydroxyvitamin D3-1alpha-hydroxylase, 25-hydroxyvitamin-D3-1alpha-hydroxylase, CYB27B1, CYP1alpha, CYP27A, CYP27B1, cytochrome P450 25-hydroxyvitamin D3-1alpha-hydroxylase, EC 1.14.13.13, extra-renal 25-hydroxyvitamin D-1alpha-hydroxylase, extra-renal 25-hydroxyvitamin D3 1alpha-hydroxylase, hydroxylase, 25-hydroxcholecalciferol 1-, More, oxygenase, 25-hydroxycholecalciferol 1-mono-, P450 cytochrome 25-hydroxyvitamin D3 1alpha-hydroxylase, VD3 25-hydroxylase, VDH, vitamin D 1alpha-hydroxylase, vitamin D hydroxylase, vitamin D3 25- and 1alpha-hydroxylase, vitamin D3 25-hydroxylase, vitamin D3 hydroxylase, [25(OH)D3]-1alpha-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.18 calcidiol 1-monooxygenase

Cloned

Cloned on EC 1.14.15.18 - calcidiol 1-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of several novel intron 2-containing, noncoding splice variant mRNAs for CYP27b1 in 1,25(OH)2D3-producing HKC-8 human proximal tubule and THP-1 monocytic cells. Noncoding splice variants of CYP27b1 are functionally active and may play a significant role in the regulation of 1,25(OH)2D3 synthesis during normal physiology
-
expressed in human kidney proximal tubular epithelial cell line HKC-8
expression in COS cells, reconstituted with recombinant cytochrome P-450, recombinant adrenodoxin, and recombinant adrenodoxin reductase
expression in COS cells, vitamin D3 25- and 1alpha-hydroxylase, DNA sequence analysis
-
expression in Escherichia coli
-
expression in Escherichia coli JM109, coexpression of adrenodoxin and NADPH-adrenodoxin reductase for reconstitution of the system
-
expression in LLC-PK1-cells
expression of His-tagged CYP27B1 in Escherichia coli strain JM109
-
expression of vitamin D hydroxylases in the VDR-/- mouse model, overview
-
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
-
expression of wild-type Vdh and mutant Vdh-K1 in Escherichia coli
-
expressionin RAW264.7 cells
-
His-tag, expressed in Rhodococcus erythropolis and Escherichia coli
-
quantitative PCR analysis of gene expression in different tissues/cells, overview
-