1.14.14.9: 4-hydroxyphenylacetate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylacetate 3-monooxygenase, go to the full flat file.

Reaction

Synonyms

4 HPA 3-hydroxyylase, 4-HPA hydroxylase, 4-hydroxyphenylacetate 3-hydroxylase, 4-hydroxyphenylacetic acid 3-hydroxylase, 4HPA 3-monooxygenase, 4HPA3H, C2-hpah, EC 1.14.13.3, HPA 3-hydroxylase, HpaB, hpaBC, HpaC, HPAH, More, p-hydroxyphenylacetate 3-hydroxylase, p-hydroxyphenylacetate hydroxylase, p-hydroxyphenylacetic 3-hydroxylase, TPY_2462, two-component p-hydroxyphenylacetate hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.9 4-hydroxyphenylacetate 3-monooxygenase

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Results	in table
4	Activating Compound
1749	AA Sequence
7	Application
1	CAS Registry Number
20	Cloned(Commentary)
72	Cofactor
8	Crystallization (Commentary)
2	General Information
3	General Stability
21	Inhibitors
31	kcat/KM [mM/s]
57	KM Value [mM]
2	Localization
5	Metals/Ions
24	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
367	Organism
7	Pathway
12	PDB ID
21	pH Optimum
4	pH Range
49	Protein Variants
17	Purification (Commentary)
20	Reaction
4	Reaction Type
71	Reference
1	Source Tissue
20	Specific Activity [micromol/min/mg]
4	Storage Stability
187	Substrates and Products (Substrate)
28	Subunits
70	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Stability [°C]
34	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, HpaC, small protein, very stable, no significant loss of activity during 2 months

Escherichia coli

-

438899

-20°C, stable for at least 5 months

Pseudomonas putida

-

438895

4°C, 15-20 days

Pseudomonas putida

-

438887

4°C, 20 mM phosphate buffer, pH 8.0, concentrated with polyethylene glycol 20000

Escherichia coli

-

438893