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1.14.14.5: alkanesulfonate monooxygenase

This is an abbreviated version!
For detailed information about alkanesulfonate monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.5

Reaction

an alkanesulfonate
+
FMNH2
 +
O2
=
an aldehyde
 +
FMN
 +
sulfite
 +
H2O

Synonyms

alkanesulfonate alpha-hydroxylase, alkanesulfonate monooxygenase, AOLE_19265, FMNH2-dependent alkanesulfonate monooxygenase, msuD, oxygenase, alkanesulfonate 1-mono-, Pfl01_3916, SsuD, SsuE, sulfate starvation-induced protein 6, YcbN

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.5 alkanesulfonate monooxygenase

Engineering

Engineering on EC 1.14.14.5 - alkanesulfonate monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C54A
-
has little effect on FMN or FMNH2 binding, kcat/Km value decreases 6fold relative to wild-type
C54S
-
has little effect on FMN or FMNH2 binding, kcat/Km value increases 3fold relative to wild-type. Is able to generate the C4a-(hydro)peroxyflavin, but the rate of formation is increased 10fold relative to wild-type
D111A
kcat/Km vlaue similar to wild-type
D251A/D252A/E253A
mutation of conserved charged amino acids, 4fold decrease in kcat/Km value
DELTA251-261
deletion of alpha-helix containing conserved charged amino acids, complete loss of activity
E20A
kcat/Km vlaue similar to wild-type
F261W
complete loss of activity
R263A
similar kinetic parameters as wild-type
R263A/R271A
similar kinetic parameters as wild-type
R271A
mutation does not alter the catalytic activity, but in the presence of reduced flavin the variant is more susceptible to proteolytic digestion compared to wild-type