1.14.14.5: alkanesulfonate monooxygenase

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For detailed information about alkanesulfonate monooxygenase, go to the full flat file.

Reaction

Synonyms

alkanesulfonate alpha-hydroxylase, alkanesulfonate monooxygenase, AOLE_19265, FMNH2-dependent alkanesulfonate monooxygenase, msuD, oxygenase, alkanesulfonate 1-mono-, Pfl01_3916, SsuD, SsuE, sulfate starvation-induced protein 6, YcbN

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.5 alkanesulfonate monooxygenase

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Results	in table
8827	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
8	Cofactor
5	Crystallization (Commentary)
2	Expression
12	General Information
12	kcat/KM [mM/s]
27	KM Value [mM]
3	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
17	Organism
4	Pathway
36	PDB ID
4	pH Optimum
10	Protein Variants
5	Purification (Commentary)
8	Reaction
1	Reaction Type
17	Reference
1	Specific Activity [micromol/min/mg]
2	Storage Stability
59	Substrates and Products (Substrate)
6	Subunits
27	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
12	Turnover Number [1/s]

Cloned

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Cloned on EC 1.14.14.5 - alkanesulfonate monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Escherichia coli

-

657786

expressed in Escherichia coli

Escherichia coli

-

438770

expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Escherichia coli

-

674310

gene msuD, genetic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Pseudomonas fluorescens

Q3K9A1

765077

gene ssuD, the alkanesulfonate monooxygenase system, expressed from the ssuEADCB operon, is comprised of a flavin reductase encoded by ssuE and monooxygenase encoded by ssuD, ssuD expressionin strain BL21(DE3)

Escherichia coli

-

672426

ligated into pET21a plasmid containing the ssuD gene, wild-type and mutants expressed in Escherichia coli BL21(DE3) super-competent cells

Escherichia coli

-

702473