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1.14.14.22: dibenzothiophene sulfone monooxygenase

This is an abbreviated version!
For detailed information about dibenzothiophene sulfone monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.22

Reaction

dibenzothiophene-5,5-dioxide
+
FMNH2
+
NADH
+
O2
=
2'-hydroxybiphenyl-2-sulfinate
+
H2O
+
FMN
+
NAD+
+
H+

Synonyms

BdsA, DBT sulfone monooxygenase, DBTO2-MO, DBTO2-monooxygenase, dibenzothiophene sulfone monooxygenase, dszA, TdsA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.22 dibenzothiophene sulfone monooxygenase

Purification

Purification on EC 1.14.14.22 - dibenzothiophene sulfone monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 102fold to homogeneity by two different steps of anion exchange chromatography, followed by hydrophobic interaction chromatography, and gel filtration
native enzyme 102fold to homogeneity from strain D-1 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, hydrophobic interaction chromatography, gel filtration
native enzyme 47.6fold by anion exchange and hydrophobic interaction chromatography, followed by another different step of anion exchange chromatography and hydroxyapatite chromatography
native enzyme 82.6fold from strain WU-S2B by three different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, gel filtration, and another step of anion exchange chromatography, recombinant enzyme from Escherichia coli 17.0fold to homogeneity by anion exchange and hydrophobic interaction chromatography, and again anion exchange chromatography
-
native enzyme from strain IGTS8 by anion exchange chromatography and gel filtration
-
native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinty chromatography
-