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1.14.14.16: steroid 21-monooxygenase

This is an abbreviated version!
For detailed information about steroid 21-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.16

Reaction

a C21 steroid
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
a 21-hydroxy-C21-steroid
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

17alpha-hydroxyprogesterone 21-hydrolase, 21-hydroxylase, 21-hydroxylase cytochrome P-450, 21OH, C3B21RA protein, CYP21, CYP21A2, CYP2D, Cytochrome P-450 specific for steroid C-21 hydroxylation, cytochrome P-450-linked mixed function oxidase system, cytochrome P-450C-21, cytochrome p450 21A2, cytochrome P450 steroid 21-hydroxylase, cytochrome P450c21, EC 1.14.1.8, EC 1.14.99.10, EC 1.99.1.11, P-450(C21), P450 oxidoreductase-21-hydroxylase, P450-C21, P450-C21B, P450c21, Progesterone 21-hydroxylase, Steroid 21-hydroxylase, steroid 21-hydroxylase system, steroid 21-hydroxylation system, steroid cytochrome P450 21-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.16 steroid 21-monooxygenase

Cloned

Cloned on EC 1.14.14.16 - steroid 21-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in COS-1 cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21-Gold DE(3) cells
expressed in Sf9 insect cell microsomes
-
expressed in Spodoptera frugiperda insect cell microsomes using a baculovirus expression system
expression of His-tagged enzyme in Escherichia coli strain JM109, coexpression with molecular chaperones GroES and GroEL
-
expression of wild-type and mutant enzymes in yeast microsomes
-
gene CYP11B!, DNA and amino acid sequence determination and analysis, genotyping
-
gene CYP21, expression in Schizosaccharomyces pombe strain CAD18, method optimization and evaluation, and quantification of the intracellular redox cofactor pool in transformed cells, overview
gene CYP21A2 or C3B21RA, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged wild-type enzyme and mutants in Escherichia coli
gene CYP21A2, DNA and amino acid sequence determination and analysis, genotyping. Recombinant expression of mutant H365Y/R356W in HEK-293T cells
-
gene CYP21A2, genotyping, expression of wild-type and mutant enzymes in HEK-293T and hepatoblastoma C3A cells, the H365Y enzyme is produced in more variable amounts than wild-type CYP21A2
-
mutant enzymes are expressed in COS-7 cells
-
mutant enzymes are expressed in Saccharomyces cerevisiae strain W303B
-
recombinant expression in yeast microsomes
-