1.14.13.92: phenylacetone monooxygenase

This is an abbreviated version!
For detailed information about phenylacetone monooxygenase, go to the full flat file.

Reaction

Synonyms

4-hydroxyacetophenone monooxygenase, Baeyer-Villiger monooxygenase, BVMO, EtaA, HAPMO, M-PAMO, More, PAMO, phenylacetone monooxygenase, Tf PAMO

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.92 phenylacetone monooxygenase

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Results	in table
3	Activating Compound
413	AA Sequence
9	Application
1	CAS Registry Number
13	Cloned(Commentary)
38	Cofactor
2	Crystallization (Commentary)
9	General Information
2	General Stability
16	Inhibitors
42	kcat/KM [mM/s]
69	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
69	Organism
13	PDB ID
16	pH Optimum
3	pH Range
1	Posttranslational Modification
112	Protein Variants
10	Purification (Commentary)
7	Reaction
2	Reaction Type
29	Reference
3	Specific Activity [micromol/min/mg]
131	Substrates and Products (Substrate)
4	Subunits
49	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
62	Turnover Number [1/s]

Organic Solvent Stability

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Organic Solvent Stability on EC 1.14.13.92 - phenylacetone monooxygenase

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2-pentanol

Thermobifida fusca

-

as a sacrificial substrate

671682

cyclohexane

Thermobifida fusca

-

stabilizes the enzyme, best organic solvent, inactivation of the enzyme within 5 min

671682

isopropanol

Thermobifida fusca

-

the most effective stoichiometric sacrificial electron donor, optimal at 5% v/v

671682

Methanol

Thermobifida fusca

-

methanol induces a significant increase of enzyme activity (up to 5fold), which is optimal at 20% (v/v)

727379

methyl tert-butyl ether

Thermobifida fusca

-

stabilizes the enzyme, inactivation of the enzyme within below 5 min

671682

additional information

Thermobifida fusca

-

the initial activity of wild type enzyme is not affected by 10% (v/v) 1,4-dioxane

726793