1.14.13.92: phenylacetone monooxygenase

This is an abbreviated version!
For detailed information about phenylacetone monooxygenase, go to the full flat file.

Reaction

Synonyms

4-hydroxyacetophenone monooxygenase, Baeyer-Villiger monooxygenase, BVMO, EtaA, HAPMO, M-PAMO, More, PAMO, phenylacetone monooxygenase, Tf PAMO

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.92 phenylacetone monooxygenase

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Results	in table
3	Activating Compound
413	AA Sequence
9	Application
1	CAS Registry Number
13	Cloned(Commentary)
38	Cofactor
2	Crystallization (Commentary)
9	General Information
2	General Stability
16	Inhibitors
42	kcat/KM [mM/s]
69	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
69	Organism
13	PDB ID
16	pH Optimum
3	pH Range
1	Posttranslational Modification
112	Protein Variants
10	Purification (Commentary)
7	Reaction
2	Reaction Type
29	Reference
3	Specific Activity [micromol/min/mg]
131	Substrates and Products (Substrate)
4	Subunits
49	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
1	Temperature Range [°C]
6	Temperature Stability [°C]
62	Turnover Number [1/s]

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Cloned on EC 1.14.13.92 - phenylacetone monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Thermobifida fusca

-

728163

BVMO gene, functional expression in Escherichia coli strains JM109 and BL21(DE3) as soluble protein

Pseudomonas putida

-

678885

expressed in Escherichia coli BL21(DE3) cells

Thermobifida fusca

-

726793

expressed in Escherichia coli Top10 cells

Thermobifida fusca

-

698487

expression in Escherichia coli

3 entries

expression of His-tagged wild-type and mutant enzymes in Escherichia coli

Thermobifida fusca

Q47PU3

685231

expression of His-tagged wild-type and mutant PAMOs in Escherichia coli strain TOP10

Thermobifida fusca

-

710979

gene pamO, recombinant expression of His-tagged enzyme in three different forms (C, N, and L) concerning the position of His-tag in Escherichia coli strain BL21(DE3), the His-tag locates at the N- and C-termini of the enzyme (PAMO N and PAMO C, respectively), PAMO L describes a construct with the His-tag distanced from the enzyme C-terminus with an additional 19-amino acid sequence, the enzyme forms do not show great differences in catalytic activity. No use of the arabinose promoter in the expression system

Thermobifida fusca

Q47PU3

764116

overexpressed in Escherichia coli

Thermobifida fusca

-

697754

overexpressed via plasmid in Escherichia coli

Pseudomonas fluorescens

-

697754

recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli

Thermobifida fusca

Q47PU3

764264

expression in Escherichia coli

Mycobacterium tuberculosis

-

659371

expression in Escherichia coli

Thermobifida fusca

-

657625

expression in Escherichia coli

Thermobifida fusca

Q47PU3

660361