1.14.13.83: precorrin-3B synthase

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For detailed information about precorrin-3B synthase, go to the full flat file.

Reaction

Synonyms

Bmei0715, CobG, CobZ, precorrin-3A monooxygenase, precorrin-3X synthase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.83 precorrin-3B synthase

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Results	in table
1662	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
3	Cofactor
3	General Information
1	General Stability
6	Metals/Ions
7	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
11	Organism
5	Pathway
19	Protein Variants
5	Purification (Commentary)
2	Reaction
11	Reference
1	Specific Activity [micromol/min/mg]
22	Substrates and Products (Substrate)
17	Synonyms
1	Systematic Name

Cloned

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Cloned on EC 1.14.13.83 - precorrin-3B synthase

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Brucella melitensis cobG is PCR-amplified, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside

Brucella melitensis

Q8YHT1

698972

expressed in Escherichia coli BL21DE3

Pseudomonas denitrificans (nom. rej.)

15269

gene cobG heterologously expressed in Escherichia coli

Pseudomonas denitrificans (nom. rej.)

650818

genetic and sequence analysis

Pseudomonas denitrificans (nom. rej.)

15270

overexpressed in recombinant strains of Escherichia coli

Pseudomonas denitrificans (nom. rej.)

3209

Pseudomonas denitrificans cobG is PCR-amplified in plasmid pCR427 and cloned into pET14b, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside

Pseudomonas denitrificans (nom. rej.)

698972