1.14.13.81: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase
This is an abbreviated version! For detailed information about magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, go to the full flat file.
gene OsCRD1, encoding a putative subunit of enzyme MPEC, is a single-copy gene in rice, sequence comparisons, quantitative real-time RT-PCR enzyme expression analysis
gene PeMPEC, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant overexpression in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV310 transformation method. Gene PeMPEC driven by the CAMV 35S promoter is transferred into the Crd1 mutant leading to functional complementation of the mutant, the chlorophyll concentration of sense plants is 22-50% higher than that of the Crd1 mutant, and 7-20% higher than that of wild-type Col-0
gene Xantha-I, recombinant expression of active XanL strictly requires co-expression with an additional protein, Ycf54, recombinant expression of His-tagegd XanL and Ycf54 in Escherichia coli strain Artic Express (DE3)
gene ygl8 encodes a catalytic subunit of MgPME cyclase, DNA and amino acid sequence determination and analysis, map-based cloning, sequence comparisons and phylogenetic analysis. Transient expression in Nicotiana benthamiana. The recombinant construct pC1305-YGL8 is introduced into the ygl8 rice mutant by Agrobacterium tumefaciens EHA105-mediated transformation. Quantitative real-time PCR expression analysis
gene ysl1, DNA and amino acid sequence determination and analysis, genotyping, sequence comparisons and phylogenetic tree, complementation of the ysl1 mutant by complementation construct pC2300-YSL1 using the Agrobacterium tumefaciens transfection method, semiquantitative and quantitative real-time RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in rice protoplasts
PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method