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1.14.13.7: phenol 2-monooxygenase (NADPH)

This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.7

Reaction

phenol
+
NADPH
+
H+
+
O2
=
catechol
+
NADP+
+
H2O

Synonyms

DmpLNO, flavin containing monooxygenase, LmPH, Mph, MphN, multi-component phenol hydroxylase, multicomponent PH, multicomponent phenol hydroxylase, multicomponent phenol hydroxylase alpha subunit, NCgl2588, oxygenase, phenol 2-mono-, PHE, phenol hydroxylase, phenol o-hydroxylase, PHH, phhY, PHIND, PHO, PHR, single-component PH, SPH

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.7 phenol 2-monooxygenase (NADPH)

Inhibitors

Inhibitors on EC 1.14.13.7 - phenol 2-monooxygenase (NADPH)

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
slight inhibition at 0.0005 to 0.001 mM
2-Fluorophenol
-
substrate inhibition
3-chlorophenol
-
substrate inhibition
3-Fluorophenol
-
substrate inhibition
4-Chlorophenol
-
substrate inhibition
4-Fluorophenol
-
substrate inhibition
5,5'-dithiobis(2-nitrobenzoate)
-
-
accessory component PHK of phenol hydroxylase
-
the accessory component of the phenol hydroxylase mediates inhibition of phenol hydroxylase activity, overview
-
acetate
-
50% inhibition at 0.123 M
Ag+
-
74.36% inhibition at 0.03 mM
ammonium sulfate
-
-
ascorbate
-
52% inhibition at 50 mM
Br-
-
50% inhibition at 0.072 M
catechol
-
substrate inhibition
CN-
-
50% inhibition at 0.004 M
Co2+
-
63% residual activity at 1 mM
copper-chelating agents
-
dithiothreitol
-
dithiothreitol acts as H2O2 generator and inhibits the oxygenase component of the enzyme, catalase protects the loss of activity
ethylene glycol
-
-
ethynylbenzene
-
reversible, competitive inhibition at concentrations above 1 mM
F-
-
50% inhibition at 0.01 M
Fe3+
-
3% residual activity at 1 mM
Glutardialdehyde
-
-
glutathione
-
-
Guanidinium chloride
-
70-80% inhibition at 0.1 M
Hg2+
-
82.05% inhibition at 0.03 mM
I-
-
50% inhibition at 0.05 M
Ni2+
-
4% residual activity at 1 mM
NO3-
-
50% inhibition at 0.035 M
p-chloromercuribenzoate
-
inhibition is reversed by dithiothreitol
p-hydroxymercuribenzoate
-
-
Phenanthroline
phenol
phosphate
-
60-70% inhibition at 5 mM
potassium ethylxanthate
-
1 mM
pyridoxal 5'-phosphate
-
reversible, 50% loss of activity in 2 min at 0.5 mM
pyridoxamine phosphate
-
slight
resorcinol
-
substrate inhibition
Sodium arsenate
Sodium borohydride
-
-
Sodium diethyldithiocarbamate
-
1 mM
Sodium dithionite
-
-
trichloroacetate
-
complete inhibition at 0.1 M
Zn2+
-
13% residual activity at 1 mM
additional information
-