1.14.13.69: alkene monooxygenase
This is an abbreviated version!
For detailed information about alkene monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.69
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1.14.13.69
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epoxidation
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xanthobacter
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vinyl
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diiron
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nocardioides
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4-monooxygenase
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propyne
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dinuclear
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rhodochrous
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smmos
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epoxygenation
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epoxyethane
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corallinus
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autotrophicus
- 1.14.13.69
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epoxidation
- xanthobacter
-
vinyl
-
diiron
- nocardioides
-
4-monooxygenase
- propyne
-
dinuclear
- rhodochrous
-
smmos
-
epoxygenation
- epoxyethane
- corallinus
- autotrophicus
Reaction
Synonyms
AkMO, alkene epoxygenase, alkene monooxygenase, AMO, ethene MO, EtnABCD, EtnC, etnD, IsoMO, isoprene monooxygenase, More, PmoABCD, propene MO, propene monooxygenase, XAMO
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Purification
Purification on EC 1.14.13.69 - alkene monooxygenase
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recombinant StrepII-tagged oxygenase component IsoABE from Rhodococcus sp. strain AD45 and recombinant N-terminally His6-tagged IsoC from Escherichia coli both by affinity chromatography and gel filtration, the latter to over 90% purity. Recombinant StrepII-tagged IsoD from Escherichia coli strain Rosetta2 by affinity, anion exchange, and hydrophobic interaction chromatography, and gel filtration, the N-terminal methionine of IsoD has been removed
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