1.14.13.5: imidazoleacetate 4-monooxygenase

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For detailed information about imidazoleacetate 4-monooxygenase, go to the full flat file.

Reaction

Synonyms

EC 1.14.1.5, imidazoleacetate hydroxylase, imidazoleacetic hydroxylase, imidazoleacetic monooxygenase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.5 imidazoleacetate 4-monooxygenase

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Results	in table
1	CAS Registry Number
20	Cofactor
5	Crystallization (Commentary)
30	Inhibitors
16	KM Value [mM]
5	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
6	Organism
4	Pathway
2	pH Optimum
4	Purification (Commentary)
1	Reaction
3	Reaction Type
6	Reference
4	Specific Activity [micromol/min/mg]
4	Storage Stability
22	Substrates and Products (Substrate)
4	Synonyms
1	Systematic Name
4	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.5 - imidazoleacetate 4-monooxygenase

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partial, using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50

Pseudomonas sp.

390101

using protamine sulfate treatment, ammonium sulfate fractionation, chromatography on TEAE-cellulose column, calcium phosphate gel treatment and chromatography on DEAE-Sephadex column

Pseudomonas sp.

390103, 390104, 390106