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1.14.13.39: nitric-oxide synthase (NADPH)

This is an abbreviated version!
For detailed information about nitric-oxide synthase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.39

Reaction

2 L-arginine + 2 NADPH + 2 H+ + 2 O2 = 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O

Synonyms

bacterial nitric oxide synthase, bacterial nitric-oxide synthase, bNOS, bsNOS, cb-NOS, cytokine inducible NOS, DNOS, e-NOS, EC-NOS, endothelial nitric oxide synthase, endothelial nitric-oxide synthase, endothelial NO synthase, endothelial NOS, endothelium-derived relaxation factor-forming enzyme, endothelium-derived relaxing factor synthase, eNOS, i-NOS, inducible nitric oxide synthase, inducible nitric-oxide synthase, inducible NO synthase, inducible NOS, iNOS, mitochondrial NO synthase, mitochondrial-specific nitric oxide synthase, mtNOS, n-NOS, NADPH diaphorase, NADPH-d, NADPH-diaphorase, NADPHd, neuronal nitric oxide synthase, neuronal nitric-oxide synthase, neuronal NO synthase, neuronal NOS, nicotinamide adenine dinucleotide phosphate-diaphorase, nitric oxid synthase, nitric oxide synthase, nitric oxide synthase-like protein, nitric oxide synthetase, nitric-oxide synthase, nNOS, nNOSalpha, NO synthase, NO synthase type I, NO synthase type II, NO synthase type III, NO-synthase, NOS, NOS I, NOS-2, NOS1, NOS2, NOS3, synthetase, nitric oxide

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.39 nitric-oxide synthase (NADPH)

Molecular Weight

Molecular Weight on EC 1.14.13.39 - nitric-oxide synthase (NADPH)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
112000
-
oxygenase subunit domain, gel filtration
115000
-
x * 120000 + x * 115000, SDS-PAGE
120000
130000
-
2 * 130000, SDS-PAGE
134000
-
x * 134000
135000
15000
x * 15000, SDS-PAGE
150000
152000
160000
178000
-
isozyme nNOS, gel filtration
19000
-
gel filtration
200000
250000
-
gel filtration
260000
-
gel filtration
300000
320000
-
gel filtration
35000
-
x * 35000, recombinant GST-tagged truncated enzyme, SDS-PAGE
43000
-
x * 43000, recombinant His6-tagged enzyme, SDS-PAGE
55000
-
oxygenase subunit domain, gel filtration
56000
-
2 * 56000, oxygenase subunit domain, SDS-PAGE
64000
-
1 * 67000 + 1 * 64000, SDS-PAGE
67000
-
1 * 67000 + 1 * 64000, SDS-PAGE
69000
-
2 * 69000, iNOS, SDS-PAGE
74000
-
reductase subunit domain, gel filtration
75000
2 * 75000, nNOS, SDS-PAGE
89000
-
2 * 89000, nNOS, SDS-PAGE, bands of 47 kDa and 29 kDa also occur
additional information
-
dissociates irreversibly into subunits in the absence of L-arginine, FAD and tetrahydrobiopterin