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1.14.13.35: anthranilate 3-monooxygenase (deaminating)

This is an abbreviated version!
For detailed information about anthranilate 3-monooxygenase (deaminating), go to the full flat file.

Word Map on EC 1.14.13.35

Reaction

anthranilate
+
NADPH
+
H+
+
O2
=
2,3-Dihydroxybenzoate
+
NADP+
+
NH3

Synonyms

anthranilate 2,3-dioxygenase (deaminating), anthranilate 2,3-hydroxylase (deaminating), anthranilate hydroxylase, anthranilate hydroxylase (deaminating), anthranilic hydroxylase, EC 1.14.12.2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.35 anthranilate 3-monooxygenase (deaminating)

Purification

Purification on EC 1.14.13.35 - anthranilate 3-monooxygenase (deaminating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, using centrifugation, protamine sulfate treatment, treatment with diethylaminoethyl-cellulose and filtration through a Buchner funnel
-
partial, using heat treatment, DEAE-cellulose column chromatography and elution with a linear gradient of 0 to 0.1 M KCl in phosphate buffer
-
partial, using protamine sulfate treatment, DEAE-cellulose treatment, alumina C-gamma treatment and hydroxylapatite treatment
-
using ammonium sulfate precipitation, column chromatography on DE23-cellulose, phenyl-Sepharose and S-300 Sephacryl
-
using protamine sulfate treatment, DEAE-cellulose treatment and alumina C-gamma treatment
-
using protamine sulfate treatment, DEAE-cellulose treatment, ammonium sulfate precipitation, fractionation on Biogel P-100 column, successive negative adsorption on alumina-gel, tricalcium phosphate gel and DEAE-cellulose column, and positive adsorption on a DEAE-Sephadex A-50 column
-