Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.13.29: 4-nitrophenol 2-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrophenol 2-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.29

Reaction

4-nitrophenol
+
NADH
 +
H+
 +
O2
=
4-nitrocatechol
 +
NAD+
 +
H2O

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, 4-nitrophenol hydroxylase, 4-nitrophenol-2-hydroxylase, 4-NPH, CYP 2E1, CYP2A/2E1, CYP2E1, cytochrome P-450 2E1, cytochrome P-450 isozyme 3a, NphA1, oxygenase, 4-nitrophenol 2-mono-, p-nitrophenol 2-hydroxylase, p-nitrophenol hydroxylase, p-nitrophenol hydroxylase component A, p-nitrophenol-hydroxylase, PNP 2-monooxygenase, PNP monooxygenase, PnpA1, PNPH, PPH, two-component 4-nitrophenol hydroxylase, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.29 4-nitrophenol 2-monooxygenase

Substrates Products

Substrates Products on EC 1.14.13.29 - 4-nitrophenol 2-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-chloro-4-nitrophenol + NADH + H+ + O2
2-chloro-4-nitrocatechol + NAD+ + H2O
show the reaction diagram
2-chloro-4-nitrophenol + NADPH + O2
1,2,4-trihydroxybenzene + ?
show the reaction diagram
3-nitrophenol + NADH + H+ + O2
4-nitrocatechol + NAD+ + H2O
show the reaction diagram
1 mM NADH, 5 M FAD, 1 mM DTT, NphA1 (approximately 0.5 mg of purified protein), and NphA2 (approximately 0.1 mg of purified protein) in 1 ml of 50 mM Tris-HCl buffer, pH 8.0, slow degradation of substrate
-
-
?
3-nitrophenol + NADH + O2
?
show the reaction diagram
-
-
-
-
?
4-chlorophenol + NADH + H+ + O2
4-chlorocatechol + NAD+ + H2O
show the reaction diagram
1 mM NADH, 5 M FAD, 1 mM DTT, NphA1 (approximately 0.5 mg of purified protein), and NphA2 (approximately 0.1 mg of purified protein) in 1 ml of 50 mM Tris-HCl buffer, pH 8.0
-
-
?
4-nitrocatechol + NADH + H+ + O2
4-nitrobenzene-1,2,3-triol + NAD+ + H2O
show the reaction diagram
4-nitrocatechol + NADPH + H+ + O2
4-nitrobenzene-1,2,3-triol + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
4-nitrophenol + NAD(P)H + H+ + O2
4-nitrocatechol + NAD(P)+ + H2O
show the reaction diagram
4-nitrophenol + NAD(P)H + O2
4-nitrocatechol + NAD(P)+ + H2O
show the reaction diagram
-
-
-
-
?
4-nitrophenol + NADH + H+ + O2
4-nitrocatechol + NAD+ + H2O
show the reaction diagram
4-nitrophenol + NADH + O2
4-nitrocatechol + NAD+ + H2O
show the reaction diagram
4-nitrophenol + NADPH + H+ + O2
4-nitrocatechol + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
4-nitrophenol + NADPH + O2
4-nitrocatechol + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
chlorzoxazone + NADH + O2
?
show the reaction diagram
p-nitrophenol + NADH + H+ + O2
p-nitrocatechol + NAD+ + H2O
show the reaction diagram
-
-
-
-
?
p-nitrophenol + NADPH + H+ + O2
p-nitrocatechol + NADP+ + H2O
show the reaction diagram
-
200 microM substrate, 0.4 mg microsomal protein, 1 mM NADPH
-
-
?
phenol + NADH + H+ + O2
catechol + NAD+ + H2O
show the reaction diagram
1 mM NADH, 5 M FAD, 1 mM DTT, NphA1 (approximately 0.5 mg of purified protein), and NphA2 (approximately 0.1 mg of purified protein) in 1 ml of 50 mM Tris-HCl buffer, pH 8.0
-
-
?
additional information
?
-