1.14.13.29: 4-nitrophenol 2-monooxygenase

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For detailed information about 4-nitrophenol 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, 4-nitrophenol hydroxylase, 4-nitrophenol-2-hydroxylase, 4-NPH, CYP 2E1, CYP2A/2E1, CYP2E1, cytochrome P-450 2E1, cytochrome P-450 isozyme 3a, NphA1, oxygenase, 4-nitrophenol 2-mono-, p-nitrophenol 2-hydroxylase, p-nitrophenol hydroxylase, p-nitrophenol hydroxylase component A, p-nitrophenol-hydroxylase, PNP 2-monooxygenase, PNP monooxygenase, PnpA1, PNPH, PPH, two-component 4-nitrophenol hydroxylase, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.29 4-nitrophenol 2-monooxygenase

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Results	in table
4	Activating Compound
57	AA Sequence
1	Application
1	CAS Registry Number
4	Cloned(Commentary)
17	Cofactor
2	Crystallization (Commentary)
131	Disease/ Diagnostics
28	General Information
1	General Stability
4	IC50 Value
27	Inhibitors
2	Ki Value [mM]
4	KM Value [mM]
11	Localization
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
26	Organism
3	Pathway
7	pH Optimum
21	Protein Variants
5	Purification (Commentary)
4	Reaction
3	Reaction Type
23	Reference
16	Source Tissue
15	Specific Activity [micromol/min/mg]
2	Storage Stability
45	Substrates and Products (Substrate)
10	Subunits
40	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.29 - 4-nitrophenol 2-monooxygenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

cells centrifuged, washed with TD buffer (50 mM Tris-HCl, 1 mM dithiothreitol (DTT), pH 7.6), centrifuged, bacterial pellet resuspended in same buffer, sonicated, centrifuged, supernatant used as cell extract or further purified with ion-exchange chromatography with a HiTrap Q-Sepharose column, active fractions are desalted with PD-10 desalting column, concentrated with Vivaspin concentrator

Rhodococcus sp. PN1

Q8RQQ0

698597

liver is homogenized, centrifuged, supernatant mixed with 8 mM calcium chloride, microsomes separated by centrifugation, pellet resuspended in 50 mM Tris-HCl with 0.1 mM ethylendiaminetetraacetic acid and 20% glycerol, pH 7.4

Sus scrofa

-

695586

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta(DE3)/pLysS by nickel affinity chromatography, gel filtration, and ultrafiltration

Rhodococcus opacus

-

763974

-

Homo sapiens

-

438908

-

Oryctolagus cuniculus

-

438903