1.14.13.24: 3-hydroxybenzoate 6-monooxygenase
This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 6-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.24
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1.14.13.24
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gentisate
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flavoprotein
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flavin
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fad
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rhodococcus
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salicylate
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hydroxylases
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jostii
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2,5-dihydroxybenzoate
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maleylpyruvate
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para-hydroxylation
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alcaligenes
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nadh-dependent
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flavin-dependent
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ncimb
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near-uv
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fumarylpyruvate
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nadh-specific
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p-hydroxybenzoate
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regioselective
- 1.14.13.24
- gentisate
- flavoprotein
- flavin
- fad
- rhodococcus
- salicylate
- hydroxylases
- jostii
- 2,5-dihydroxybenzoate
- maleylpyruvate
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para-hydroxylation
- alcaligenes
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nadh-dependent
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flavin-dependent
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ncimb
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near-uv
- fumarylpyruvate
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nadh-specific
- p-hydroxybenzoate
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regioselective
Reaction
Synonyms
3-HBA-6-hydroxylase, 3-hydroxybenzoate 6-hydroxylase, 3-hydroxybenzoate-6-hydroxylase, 3-hydroxybenzoic acid-6-hydroxylase, 3HB6H, m-hydroxybenzoate 6-hydroxylase, MNX2, Mnx2p, NagX, NarX, Ncgl2923, oxygenase, 3-hydroxybenzoate 6-mono-, XlnD
ECTree
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Cofactor
Cofactor on EC 1.14.13.24 - 3-hydroxybenzoate 6-monooxygenase
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FAD
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1 mol FAD per mol enzyme, exogenous addition of FAD required to regenerate oxidized FAD during catalysis
FAD
3fold activation at 0.015 mM. Sequence analysis of FAD/NADPH-binding domain of Ncgl2923, overview
FAD
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residues 305, 306 and 308 are important for FAD binding. FAD concentration in the purified 3HB6H (0.29 mM) is 0.30 mM, and FAD/enzyme ratio is 1.02/0.05 determined by HPLC analysis
NADH
XlnD can utilize either NADH or NADPH as the electron donor, NADH is preferred
NADPH
XlnD can utilize either NADH or NADPH as the electron donor, NADH is preferred
the enzyme harbors a phospholipid ligand. The purified enzyme obtained from expressing the gene encoding 3HB6H from Rhodococcus jostii RHA1 in the host Escherichia coli contains a mixture of phosphatidylglycerol and phosphatidylethanolamine, which are the major constituents of the cytoplasmic membrane of Escherichia coli. The enzyme that is produced in the host Rhodococcus jostii RHA#2 by employing a newly developed actinomycete expression system, contains phosphatidylinositol, which is a specific constituent of actinomycete membranes. The lipid cofactor stabilizes monomer-monomer contact
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additional information
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the enzyme harbors a phospholipid ligand. The purified enzyme obtained from expressing the gene encoding 3HB6H from Rhodococcus jostii RHA1 in the host Escherichia coli contains a mixture of phosphatidylglycerol and phosphatidylethanolamine, which are the major constituents of the cytoplasmic membrane of Escherichia coli. The enzyme that is produced in the host Rhodococcus jostii RHA#2 by employing a newly developed actinomycete expression system, contains phosphatidylinositol, which is a specific constituent of actinomycete membranes. The lipid cofactor stabilizes monomer-monomer contact
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