Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.13.24: 3-hydroxybenzoate 6-monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 6-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.24

Reaction

3-hydroxybenzoate
+
NADH
 +
H+
 +
O2
=
2,5-Dihydroxybenzoate
 +
NAD+
 +
H2O

Synonyms

3-HBA-6-hydroxylase, 3-hydroxybenzoate 6-hydroxylase, 3-hydroxybenzoate-6-hydroxylase, 3-hydroxybenzoic acid-6-hydroxylase, 3HB6H, m-hydroxybenzoate 6-hydroxylase, MNX2, Mnx2p, NagX, NarX, Ncgl2923, oxygenase, 3-hydroxybenzoate 6-mono-, XlnD

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.24 3-hydroxybenzoate 6-monooxygenase

Cloned

Cloned on EC 1.14.13.24 - 3-hydroxybenzoate 6-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Top10 cells
expression in Rhodococcus jostii RHA#2
gene 3HB6H is encoded in a cluster that potentially encodes for gentisic acid degradation, sequence comparisons and phylogenetic analysis and tree recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene mhbM, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
gene narX, DNA and amino acid sequence determination and analysis of the gentisate catabolic gene cluster. Among the 13 complete open reading frames deduced from this fragment, three narIKL encode the enzymes involved in the reactions of gentisate catabolism, of these, NarX is hydroxybenzoate 6-monooxygenase which converts 3-hydroxybenzoate to 2,5-dihydroxybenzoate. The narX gene is divergently transcribed with narIKL. Sequence comparisons, overview. Expression in of the His-tagged NarX in Escherichia coli strain Rosetta (DE3) pLysS
gene ncgl2923, sequence determination, analysis, and comparisons, functional overexpression in of N-terminally His-tagged Ncgl2923 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene xlnD, DNA and amino acid sequence determination and analysis, subcloning and overexpression of the His-tagged enzyme in Escherichia coli strain DH5alpha, S17-1, and BL21(DE3)
orf2 or gene nagX, which does not encode salicylate 5-hydroxylase, but 3-hydroxybenzoate 6-hydroxylase, DNA and amino acid sequence determination and analysis, genetic organization, overview, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-