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1.14.13.20: 2,4-dichlorophenol 6-monooxygenase

This is an abbreviated version!
For detailed information about 2,4-dichlorophenol 6-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.20

Reaction

2,4-Dichlorophenol
+
NADPH
+
H+
+
O2
=
3,5-dichlorocatechol
+
NADP+
+
H2O

Synonyms

2,4-DCP hydroxylase, 2,4-dichlorophenol hydroxylase, 2,4-dichlorophenol monooxygenase, chlorophenol hydroxylase, DCM, oxygenase, 2,4-dichlorophenol 6-mono, oxygenase, 2,4-dichlorophenol mono-, phenol hydroxylase, tfdB, TfdB-JLU

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.20 2,4-dichlorophenol 6-monooxygenase

Cloned

Cloned on EC 1.14.13.20 - 2,4-dichlorophenol 6-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
dcm gene clusters, dcmAE and dcmB1B2CD, are successfully cloned from Rhodococcus sp. strain JT-3
-
expression in Escherichia coli
Soil bacterium
-
expression in Pseudomonas putida
-
gene tfdBa, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21, subcloning in Escherichia coli strains JM109 amd T7 Blue
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha
-