1.14.13.2: 4-hydroxybenzoate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.2

1.14.13.2

flavin

fad

fluorescens

flavoproteins

isoalloxazine

protocatechuate

3,4-dioxygenase

massey

2,4-dihydroxybenzoate

ballou

c4a-hydroperoxide

3-hydroxybenzoate

gatti

degradation

analysis

Reaction

Synonyms

4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-HBA-3-hydroxylase, 4-HBMO, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase, 4-hydroxybenzoate hydroxylase, 4-hydroxybenzoate monooxygenase, 4-hydroxybenzoic hydroxylase, 4HBA 3-hydroxylase, An_PhhA, BxeA2040, FAD-dependent 4-hydroxybenzoate hydroxylase, fungal 4-hydroxybenzoate 3-hydroxylase, HBH, m-hydroxybenzoate hydroxylase, MobA, NADPH-dependent 4-HBA hydroxylase, ncgl1032, oxygenase, 4-hydroxybenzoate 3-mono-, p-hydroxybenzoate hydroxylase, p-hydroxybenzoate-3-hydroxylase, p-hydroxybenzoic acid hydrolase, p-hydroxybenzoic acid hydroxylase, p-hydroxybenzoic hydroxylase, PaPobA, para-hydroxybenzoate hydroxylase, PHBAD, PHBH, PHBHase, PHBHCn2, phhA, PobA, pobACg, POHBase, Reut_B4006

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.2 4-hydroxybenzoate 3-monooxygenase

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Results	in table
8	Activating Compound
4541	AA Sequence
5	Application
1	CAS Registry Number
22	Cloned(Commentary)
96	Cofactor
17	Crystallization (Commentary)
6	Expression
43	General Information
7	General Stability
88	Inhibitors
17	kcat/KM [mM/s]
13	Ki Value [mM]
88	KM Value [mM]
1	Metals/Ions
27	Molecular Weight [Da]
57	Natural Substrates/ Products (Substrates)
170	Organism
14	Pathway
37	PDB ID
17	pH Optimum
1	pH Range
4	pH Stability
147	Protein Variants
19	Purification (Commentary)
14	Reaction
3	Reaction Type
115	Reference
16	Specific Activity [micromol/min/mg]
5	Storage Stability
208	Substrates and Products (Substrate)
32	Subunits
145	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
19	Temperature Stability [°C]
53	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.2 - 4-hydroxybenzoate 3-monooxygenase

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8 entries

microheterogeneity of the highly purified enzyme. Different form of enzyme molecules are due to the partial oxidation of Cys116 in the sequence of the enzyme

Pseudomonas fluorescens

390030

recombinant enzyme

Acinetobacter calcoaceticus

390038

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration, followed by dialysis and ultrafiltration

Pseudomonas putida

Q88H28

763846

recombinant PHBH

Pseudomonas aeruginosa

658048

recombinat enzyme, cloned in Escherichia coli

Pseudomonas fluorescens

390042