1.14.13.2: 4-hydroxybenzoate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.2

1.14.13.2

flavin

fad

fluorescens

flavoproteins

isoalloxazine

protocatechuate

3,4-dioxygenase

massey

2,4-dihydroxybenzoate

ballou

c4a-hydroperoxide

3-hydroxybenzoate

gatti

degradation

analysis

Reaction

Synonyms

4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-HBA-3-hydroxylase, 4-HBMO, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase, 4-hydroxybenzoate hydroxylase, 4-hydroxybenzoate monooxygenase, 4-hydroxybenzoic hydroxylase, 4HBA 3-hydroxylase, An_PhhA, BxeA2040, FAD-dependent 4-hydroxybenzoate hydroxylase, fungal 4-hydroxybenzoate 3-hydroxylase, HBH, m-hydroxybenzoate hydroxylase, MobA, NADPH-dependent 4-HBA hydroxylase, ncgl1032, oxygenase, 4-hydroxybenzoate 3-mono-, p-hydroxybenzoate hydroxylase, p-hydroxybenzoate-3-hydroxylase, p-hydroxybenzoic acid hydrolase, p-hydroxybenzoic acid hydroxylase, p-hydroxybenzoic hydroxylase, PaPobA, para-hydroxybenzoate hydroxylase, PHBAD, PHBH, PHBHase, PHBHCn2, phhA, PobA, pobACg, POHBase, Reut_B4006

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.2 4-hydroxybenzoate 3-monooxygenase

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Results	in table
8	Activating Compound
4541	AA Sequence
5	Application
1	CAS Registry Number
22	Cloned(Commentary)
96	Cofactor
17	Crystallization (Commentary)
6	Expression
43	General Information
7	General Stability
88	Inhibitors
17	kcat/KM [mM/s]
13	Ki Value [mM]
88	KM Value [mM]
1	Metals/Ions
27	Molecular Weight [Da]
57	Natural Substrates/ Products (Substrates)
170	Organism
14	Pathway
37	PDB ID
17	pH Optimum
1	pH Range
4	pH Stability
147	Protein Variants
19	Purification (Commentary)
14	Reaction
3	Reaction Type
115	Reference
16	Specific Activity [micromol/min/mg]
5	Storage Stability
208	Substrates and Products (Substrate)
32	Subunits
145	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
19	Temperature Stability [°C]
53	Turnover Number [1/s]

Cofactor

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Cofactor on EC 1.14.13.2 - 4-hydroxybenzoate 3-monooxygenase

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1-deaza-FAD

Pseudomonas aeruginosa

when 1-deaza-FAD is used as cofactor, the enzyme carries out each step in catalysis except the transfer of oxygen to 4-hydroxybenzoate

390026

6-Hydroxy-FAD

Pseudomonas aeruginosa

when 6-hydroxy-FAD is used as cofactor, the enzyme has a lower turnover rate than the native enzyme

390026

arabinoflavin adenine dinucleotide

Pseudomonas fluorescens

like native enzyme the arabinoflavin adenine dinucleotide containing 4-hydroxybenzoate hydroxylase preferentially binds the phenolate form of the substrate. The oxidative part of the catalytic cycle of a FAD-containing 4-hydroxybenzoate hydroxylase differs from the native enzyme. Partial uncoupling of hydroxylation results in the formation of about 0.3 mol of 3,4-dihydroxybenzoate and 0.7 mol of H2O2 per mol of NADPH oxidized

FAD

NADH

NADPH

additional information

2 entries