1.14.13.181: 13-deoxydaunorubicin hydroxylase

This is an abbreviated version!
For detailed information about 13-deoxydaunorubicin hydroxylase, go to the full flat file.

Reaction

Synonyms

CYP109A2, DoxA, DoxA monooxygenase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.181 13-deoxydaunorubicin hydroxylase

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Results	in table
5	AA Sequence
3	Cloned(Commentary)
6	Cofactor
6	General Information
6	Inhibitors
4	kcat/KM [mM/s]
1	Ki Value [mM]
4	KM Value [mM]
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
10	Organism
6	Pathway
1	pH Optimum
2	Purification (Commentary)
2	Reaction
9	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
25	Substrates and Products (Substrate)
5	Subunits
11	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 1.14.13.181 - 13-deoxydaunorubicin hydroxylase

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

dithiothreitol

Streptomyces sp.

Q59971

strongly inhibitory, inhibition is reversible after desalting

722500

doxorubicin

Streptomyces sp.

Q59971

completely inhibits DoxA activity at both 1 and 5 mM, competitive with respect to daunorubicinol oxidation

722500

quinidine

Streptomyces sp.

Q59971

inhibits DoxA oxidation of daunorubicinol by 11% at 1 mM. At 5 mM, it inhibits at more than 95%

722500

sulfaphenazole

Streptomyces sp.

Q59971

inhibits DoxA oxidation of daunorubicinol by 20% at 1 mM. At 5 mM, it inhibits at more than 95%

722500

troleandomycin

Streptomyces sp.

Q59971

inhibits DoxA oxidation of daunorubicinol by 35% at 1 mM. At 5 mM, it inhibits at more than 95%

722500

additional information

Streptomyces sp.

Q59971

high ionic strength buffers containing 100 mM sodium phosphate, also strongly inhibits DoxA activity. This effect is partially reversible after exchange into low ionic strength buffers, such as 20 mM N-(2-hydroxyethyl)piperazine-N9-(2-ethanesulfonic acid) (HEPES) or 10 mM sodium phosphate (pH 7.5). No inhibition: 4-methylpyrazole (1 or 5 mM), rhodomycin D (1-5 mM)

722500

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