1.14.13.181: 13-deoxydaunorubicin hydroxylase

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For detailed information about 13-deoxydaunorubicin hydroxylase, go to the full flat file.

Reaction

Synonyms

CYP109A2, DoxA, DoxA monooxygenase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.181 13-deoxydaunorubicin hydroxylase

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Results	in table
5	AA Sequence
3	Cloned(Commentary)
6	Cofactor
6	General Information
6	Inhibitors
4	kcat/KM [mM/s]
1	Ki Value [mM]
4	KM Value [mM]
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
10	Organism
6	Pathway
1	pH Optimum
2	Purification (Commentary)
2	Reaction
9	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
25	Substrates and Products (Substrate)
5	Subunits
11	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Cloned

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Cloned on EC 1.14.13.181 - 13-deoxydaunorubicin hydroxylase

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doxA expression in Streptomyces peucetius

Streptomyces peucetius

Q9ZAU3

722501

expression in Streptomyces lividans TK24

Streptomyces sp.

Q59971

722492

recombinant overexpression of His-tagged enzyme DoxA along with its His-tagged redox partners from Streptomyces peucetius FDX1, FDR2, and FDX3, and the putidaredoxin and putidaredoxin reductase from Pseudomonas putida, that are essential equivalents of the class I type of bacterial electron-transport system, in Escherichia coli strain BL21(DE3) harboring the GroES/EL-expressing plasmid pGro7

Streptomyces peucetius

744164