1.14.13.180: aklavinone 12-hydroxylase

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For detailed information about aklavinone 12-hydroxylase, go to the full flat file.

Reaction

Synonyms

aklavinone 12-hydroxylase, DnrF, RdmE

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.180 aklavinone 12-hydroxylase

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Results	in table
47	AA Sequence
1	Application
5	Cloned(Commentary)
5	Cofactor
2	Crystallization (Commentary)
2	General Information
2	Inhibitors
4	KM Value [mM]
3	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
10	Organism
5	Pathway
1	pH Optimum
1	pI Value
5	Protein Variants
1	Purification (Commentary)
1	Reaction
10	Reference
12	Substrates and Products (Substrate)
4	Subunits
11	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Cloned

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Cloned on EC 1.14.13.180 - aklavinone 12-hydroxylase

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2 entries

expression in doxorubicin-sensitive Streptomyces galilaeus ATCC 31133. Transformation of Streptomyces galilaeus ATCC 31133 with plasmid pMC213 containing the aklavinone 11-hydroxylase gene (dnrF) results in the production of many red pigments. A new metabolite is purified, and the position of the newly introduced hydroxyl group is determined. The result indicates that the aklavinone 11-hydroxylase gene is stably expressed in Streptomyces galilaeus ATCC 31133 and that it gives rise to a hybrid aclacinomycin A which shows highly specific in vitro cytotoxicity against leukemia and melanoma cell lines

Streptomyces peucetius

721298

heterologous expression of dnrF in Streptomyces liwidans TK23 and in Escherichia coli. Since the fusion protein does not show any hydroxylase activity, the dnrF gene is cloned into the pT7-7 vector that allows the expression of an unfused DnrF protein

Streptomyces peucetius

P32009

723117

overexpression of the gene in Streptomyces lividans

Streptomyces purpurascens

721697