1.14.13.148: trimethylamine monooxygenase
This is an abbreviated version!
For detailed information about trimethylamine monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.148
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1.14.13.148
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1.14.13.8
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flavin-containing
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trimethylamine-n-oxide
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fad-containing
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trimethylaminuria
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methimazole
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n-oxygenation
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dimethylaniline
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s-oxidation
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n-octylamine
- 1.14.13.148
-
1.14.13.8
-
flavin-containing
- trimethylamine-n-oxide
-
fad-containing
-
trimethylaminuria
- methimazole
-
n-oxygenation
- dimethylaniline
-
s-oxidation
- n-octylamine
Reaction
Synonyms
dimethyl sulfoxide/trimethylamine N-oxide reductase, EC 1.14.13.8, FAD-containing monooxygenase, flavin containing monooxygenase 3, flavin monooxygenase 3, flavin-containing monooxygenase, flavin-containing monooxygenase 3, FMO, FMO3, FMO4, PB7211, TMA monooxygenase, TMA N-oxygenase, TMM
ECTree
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Engineering
Engineering on EC 1.14.13.148 - trimethylamine monooxygenase
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D314A
mutation decreases the activity significantly
D314E
enzymatic activity and the apparent KM toward NADPH are only slightly affected
E158K
E24D
the mutation impacts the structure of the Rossmann fold involved in FAD binding and does not alter FMO3 catalytic activity
E308G
K416N
the mutation has minimal impact on either hydrophilicity or protein structure
N61K
the mutation disrupts the secondary structure of a conserved membrane interaction domain and does not alter FMO3 catalytic activity
T428R
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methimazole activity of the mutant enzyme is stimulated (maximally 25% when the methimazole concentration is 2 mM) to the same extend of native enzyme up to an imipramine concentration of 3 mM. The activity of the mutant is inhibited at concentrations above 0.3 mM imipramine, 0.75 mM imipramine causes 93% inhibition of methimazole activity of the mutant enzyme. Chlorpromazine activates the mutant enzyme only at high substrate concentrations (0.1-2 mM)
V257M