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1.14.13.135: 1-hydroxy-2-naphthoate hydroxylase

This is an abbreviated version!
For detailed information about 1-hydroxy-2-naphthoate hydroxylase, go to the full flat file.

Reaction

1-hydroxy-2-naphthoate
+
NAD(P)H
+
H+
+
O2
=
1,2-dihydroxynaphthalene
+
NAD(P)+
+
H2O
+
CO2

Synonyms

1-hydroxy-2-naphthoic acid hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.135 1-hydroxy-2-naphthoate hydroxylase

Purification

Purification on EC 1.14.13.135 - 1-hydroxy-2-naphthoate hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 81fold by heat treatment at 60°C, ammonium sulfate fractionation and anion exchange chromatography. Additional purification steps, such as hydrophobic interaction chromatography or gel filtration, lead to the total or a significant, 70%, loss of activity, respectively, without achieving any further purification
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