1.14.13.113: FAD-dependent urate hydroxylase
This is an abbreviated version!
For detailed information about FAD-dependent urate hydroxylase, go to the full flat file.
Reaction
Synonyms
FAD-dependent urate oxidase, flavin adenine dinucleotide-dependent urate oxidase, HpxO, HpxO enzyme, HpyO, urate hydroxylase, XCC0279
ECTree
Advanced search results
Crystallization
Crystallization on EC 1.14.13.113 - FAD-dependent urate hydroxylase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
hanging drop vapor diffusion method, using 0.05 M KH2PO4 and 20% (w/v) PEG 8000
structures of enzyme with and without uric acid at 2.0 and 2.2 A, respectively, and of the R204Q variant at 2.0 A resolution in the absence of uric acid. The variant structure is very similar to that of wild-type HpxO except for the conformation of Arg103, which interacts with FAD in the variant but not in the wild-type structure. The R204Q variant results in the uncoupling of nicotinamide adenine dinucleotide oxidation from uric acid hydroxylation. This suggests that Arg204 facilitates the deprotonation of uric acid, activating it for the oxygen transfer