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1.14.13.113: FAD-dependent urate hydroxylase

This is an abbreviated version!
For detailed information about FAD-dependent urate hydroxylase, go to the full flat file.

Reaction

Urate
+
NADH
+
H+
+
O2
=
5-hydroxyisourate
+
NAD+
+
H2O

Synonyms

FAD-dependent urate oxidase, flavin adenine dinucleotide-dependent urate oxidase, HpxO, HpxO enzyme, HpyO, urate hydroxylase, XCC0279

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.113 FAD-dependent urate hydroxylase

Cloned

Cloned on EC 1.14.13.113 - FAD-dependent urate hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and heterologous overexpression as N-terminally six-His tagged protein
-
expressed in Acinetobacter baylyi strain ADP1
expressed in Escherichia coli