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1.14.12.22: carbazole 1,9a-dioxygenase

This is an abbreviated version!
For detailed information about carbazole 1,9a-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.22

Reaction

9H-carbazole
+
NAD(P)H
 +
H+
 +
O2
=
2'-aminobiphenyl-2,3-diol
 +
NAD(P)+

Synonyms

CarAa, CarAc, carbazole dioxygenase, CARDO, CARDO-F, CARDO-R

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.22 carbazole 1,9a-dioxygenase

Storage Stability

Storage Stability on EC 1.14.12.22 - carbazole 1,9a-dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C 10% glycerol, 8 days, more than 90% residual activity, isolated subunit CarAa
0°C 50 mM Tris-HCl pH 7.5, 24 h, 90% residual activity, reconstitued CARDO system
0°C, 10 h, full activity, and 100 h, 93% residual activiy, ferredoxin reductase subunit CarAd
0°C, 10% glycerol, 8 days, more than 90% residual activity for isolated subunit CarAa
0°C, 50 mM Tris-HCl pH 7.5, 24 h, 90% residual activity for reconstituted CARDO system
4°C, 10% glycerol, 24 h, full activity for isolated subunit CarAa
4°C, 10% glycerol, 24 h, full activity, isolated subunit CarAa