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1.14.12.18: biphenyl 2,3-dioxygenase

This is an abbreviated version!
For detailed information about biphenyl 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.14.12.18

Reaction

biphenyl
+
NADH
 +
H+
 +
O2
=
(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol
 +
NAD+

Synonyms

2,3-biphenyl dioxygenase, BDO, biphenyl 2, 3-dioxygenase, Biphenyl 2,3-dioxygenase, biphenyl dioxygenase, biphenyl-2,3-dioxygenase, BPDO, BPDOB356, BPDOCam-1, BPDOLB400, BPH, BPH dox, BphA, BphA1, BphA1A2, BphABC, BphABCD, BphAE, BPO, ThebphA1fA2f

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
                1.14.12.18 biphenyl 2,3-dioxygenase

Purification

Purification on EC 1.14.12.18 - biphenyl 2,3-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
active BphAE and BphG copurified from Nicotiana benthamiana leaves
-
butyl-Sepharose XK26/40 column chromatography, Q-Sepharose column chromatography, and hydroxyapatite column chromatography
-
His-tagged ISP, expressed in Escherichia coli
-
His-tagged ISP, expressed in Escherichia coli and Pseudomonas putida
-
purification from heterologous expression in Pseudomonas putida KT2442
-
purification of the iron sulfur protein (ISP) of biphenyl 2,3-dioxygenase
-
purification of the NADH: ferredoxin oxidoreductase component
-
Q-Sepharose FF column chromatography
recombinant His-tagged subunits alpha and beta and mutant variants from Escherichia coli strain C41 (DE3) by affinity chromatography, the tag is cleaved off by thrombin
recombinant His-tagged subunits alpha and beta and mutant variants from Escherichia coli strain C41(DE3) by affinity chromatography, the tag is cleaved off by thrombin