1.14.11.6: thymine dioxygenase

This is an abbreviated version!
For detailed information about thymine dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.6

1.14.11.6

pyrimidine

leishmania

crassa

glucosylated

neurospora

alpha-ketoglutarate

ascorbate

trypanosomes

deoxyribonucleoside

glutinis

rhodotorula

2'-hydroxylase

5-hydroxymethylcytosine

telomeric

salvage

kinetoplastids

deoxyuridine

protozoa

alpha-ketoglutarate-dependent

5-formyluracil

small-angle

Reaction

Synonyms

5-hydroxy-methyluracil dioxygenase, 5-hydroxy-methyluracil oxygenase, EC 1.14.11.5, Fe2+/2-oxoglutarate dependent hydroxylase, J binding protein 1, J binding protein 2, J-binding protein 1, J-binding protein 2, JBP1, JBP2, More, T7H, thymidine hydroxylase, thymine 7-hydroxylase, thymine dioxygenase, thymine hydroxylase, thymine-7-hydroxylase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

1.14.11.6 thymine dioxygenase

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Results	in table
13	Activating Compound
109	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
3	General Information
1	General Stability
28	Inhibitors
3	kcat/KM [mM/s]
4	Ki Value [mM]
33	KM Value [mM]
1	Localization
15	Metals/Ions
3	Molecular Weight [Da]
50	Natural Substrates/ Products (Substrates)
68	Organism
2	PDB ID
2	pH Optimum
42	Protein Variants
8	Purification (Commentary)
1	Reaction
5	Reaction Type
31	Reference
10	Source Tissue
3	Specific Activity [micromol/min/mg]
5	Storage Stability
98	Substrates and Products (Substrate)
4	Subunits
29	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
4	Temperature Stability [°C]
12	Turnover Number [1/s]

Purification

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Purification on EC 1.14.11.6 - thymine dioxygenase

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2 entries

native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration. Recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin, followed by gel filtration

partial

recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Condon Plus by nickel affinity chromatography and gel filtration

Neurospora crassa

Q7RYZ9

745921