Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.11.6: thymine dioxygenase

This is an abbreviated version!
For detailed information about thymine dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.6

Reaction

thymine
+
2-oxoglutarate
 +
O2
=
5-Hydroxymethyluracil
 +
succinate
 +
CO2

Synonyms

5-hydroxy-methyluracil dioxygenase, 5-hydroxy-methyluracil oxygenase, EC 1.14.11.5, Fe2+/2-oxoglutarate dependent hydroxylase, J binding protein 1, J binding protein 2, J-binding protein 1, J-binding protein 2, JBP1, JBP2, More, T7H, thymidine hydroxylase, thymine 7-hydroxylase, thymine dioxygenase, thymine hydroxylase, thymine-7-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.6 thymine dioxygenase

Cloned

Cloned on EC 1.14.11.6 - thymine dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21(DE3)Gold
expression in Escherichia coli, fusion protein with green fluorescent protein and with His-tag
expression of either the wild type JBP2 or the mutant JBP2 proteins in insect stage trypanosomes, which do not contain the modified base J and determined the base J content of each cell line, mutations made within the key catalytic domain ablates the ability of JBP2 to initiate de novo base J synthesis
JBP2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes, the latter contain 4 exchanges of conserved residues
recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Codon Plus