1.14.11.33: DNA oxidative demethylase
This is an abbreviated version!
For detailed information about DNA oxidative demethylase, go to the full flat file.
Word Map on EC 1.14.11.33
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1.14.11.33
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demethylation
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3-methylcytosine
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wobble
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1,n6-ethenoadenine
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alkbhs
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2-oxoglutarate-dependent
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dealkylation
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writer
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etheno
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epitranscriptomic
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5-methoxycarbonylmethyluridine
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n1-methyladenosine
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n6-methyladenine
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analysis
- 1.14.11.33
-
demethylation
- 3-methylcytosine
-
wobble
- 1,n6-ethenoadenine
-
alkbhs
-
2-oxoglutarate-dependent
-
dealkylation
-
writer
-
etheno
-
epitranscriptomic
-
5-methoxycarbonylmethyluridine
- n1-methyladenosine
- n6-methyladenine
- analysis
Reaction
Synonyms
1-methyladenine-DNA dioxygenase, ABH1, ABH2, ABH3, AlkB, AlkB homolog 1, ALKBH2, ALKBH3, ALKBH8, alkylated DNA repair protein, alpha-ketoglutarate-dependent dioxygenase ABH1, FTO
ECTree
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Engineering
Engineering on EC 1.14.11.33 - DNA oxidative demethylase
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D61A
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the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate
H59A
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the mutant is unable to rescue methylated RNA phage MS2 and is unable to convert 2-oxoglutarate to succinate
D133C
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the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme
D135A
the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0
D135I
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
D135N
D135S
the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0
E136L
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme
H131C
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the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme
H187C
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the mutant contains no iron and shows indistinguishable chromatographic behavior from that of wild type enzyme
D173A
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site-directed mutagenesis, the mutant maintains nucleolar localization, but is not able to enhance transcription from the rDNA luciferase reporter. eÉctopic expression of wild-type ABH2 leads to a 2.5fold increase of pre-rRNA, whereas ectopic expression of D173A mutant only leads to a marginal increase of pre-rRNA
H131C
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the mutant slightly prefers single-stranded DNA substrates over any double-stranded DNA
H171C
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the ABH2 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins and cross-links efficiently with the single-stranded DNA-1 and DNA3-6
H191C
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the ABH3 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins and cross-links to DNA-1 much more efficiently than the wild type protein after a 20 h incubation
H231C
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the ABH1 mutant shows indistinguishable chromatographic behaviors from those of the wild type proteins, no cross-link between the mutant protein and the single-stranded and double-stranded DNA probes is observed
the mutant displays about 5-15% of wild type activity towards 1-methyladenine at pH 7.0
D135N
the mutant shows increased activity with RNA-N6-methyladenine and reduced activity with DNA-N1-methyladenine compared to the wild type enzyme