1.14.11.26: deacetoxycephalosporin-C hydroxylase
This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C hydroxylase, go to the full flat file.
Word Map on EC 1.14.11.26
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1.14.11.26
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clavuligerus
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penicillin
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dithiothreitol
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ascorbate
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acremonium
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alpha-ketoglutarate
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chrysogenum
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ring-expansion
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n-ethylmaleimide
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expandase
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cephalosporium
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synthesis
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biotechnology
- 1.14.11.26
- clavuligerus
- penicillin
- dithiothreitol
- ascorbate
- acremonium
- alpha-ketoglutarate
- chrysogenum
-
ring-expansion
- n-ethylmaleimide
-
expandase
-
cephalosporium
- synthesis
- biotechnology
Reaction
Synonyms
cefF, Cephalosporin biosynthesis expandase/hydroxylase, DACS, DAOC hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOCS, deacetoxycephalosporin C hydroxylase, deacetoxycephalosporin C synthase, deacetylcephalosporin C synthase
ECTree
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Cloned
Cloned on EC 1.14.11.26 - deacetoxycephalosporin-C hydroxylase
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geen cefF, recombinant exression of cefF library mutants, site-directed mutants, and wild-type enzyme in Escherichia coli strain BL21(DE3)
gene cefF, subcloning in Escherichia coli, functional expression in Acremonium chrysogenum strain CGMCC3.3795 via Agrobacterium tumefaciens-mediated transformation under the promoter of pcbC, leading to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis