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1.14.11.21: clavaminate synthase

This is an abbreviated version!
For detailed information about clavaminate synthase, go to the full flat file.

Word Map on EC 1.14.11.21

Reaction

deoxyamidinoproclavaminate
+
2-oxoglutarate
+
O2
=
amidinoproclavaminate
+
succinate
+
CO2

Synonyms

CAS, CAS1, CAS2, clavaminate synthase 1, clavaminate synthase 2, clavaminic acid synthase, CS, synthase, clavaminate

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.21 clavaminate synthase

Engineering

Engineering on EC 1.14.11.21 - clavaminate synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C162L
-
isozyme CAS2, site-directed mutagenesis, same activity in hydroxylation of N-alpha-acetyl-L-arginine as the wild-type enzyme
C200L
-
isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
C201L
-
isozyme CAS2, site-directed mutagenesis, 40% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
C8L
-
isozyme CAS2, site-directed mutagenesis, 42% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H109L
-
isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H109Q
-
isozyme CS2, site-directed mutagensis, 83% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity
H122L
-
isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H122Q
-
isozyme CS2, site-directed mutagensis, 96% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity
H131L
-
isozyme CAS2, site-directed mutagenesis, 36% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H131Q
-
isozyme CS2, site-directed mutagensis, 16% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity
H145E
-
isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H145L
-
isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H145Q
H151l
-
isozyme CAS2, site-directed mutagenesis, below 1% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H151Q
-
isozyme CS2, site-directed mutagensis, 10% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity
H167L
-
isozyme CAS2, site-directed mutagenesis, 19% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H167Q
-
isozyme CS2, site-directed mutagensis, 95% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity
H280L
-
isozyme CAS2, site-directed mutagenesis, 86% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H280Q
-
isozyme CS2, site-directed mutagensis, no activity of clavaminate formation, but slight hydroxylating activity of deoxyguanidinoproclavaminate
H297L
-
isozyme CAS2, site-directed mutagenesis, 3% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
H297Q
-
isozyme CS2, site-directed mutagensis, 65% activity of clavaminate formation compared to the wild-type enzyme, unaltered hydroxylating activity
Q154L
-
isozyme CAS2, site-directed mutagenesis, 23% activity in hydroxylation of N-alpha-acetyl-L-arginine compared to the wild-type enzyme
additional information