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1.14.11.2: procollagen-proline 4-dioxygenase

This is an abbreviated version!
For detailed information about procollagen-proline 4-dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.2

Reaction

Procollagen L-proline
+
2-oxoglutarate
+
O2
=
procollagen trans-4-hydroxy-L-proline
+
succinate
+
CO2

Synonyms

A085R, alpha (I) subunit, anthrax-P4H, AT-P4H-1, At-P4H-2, BaP4H, C-P4H, C-P4H alpha subunit (III), C-P4H alpha(I), C-P4H alpha(III), CePHY-1/PHY-2/PDI2, collagen proline hydroxylase, collagen prolyl 4-hydroxylase, collagen prolyl 4-hydroxylase (type-II), collagen prolyl 4-hydroxylase 1, collagen prolyl-4-hydroxylase, collagen prolyl-4-hydroxylase alpha subunit 2, CP4H, CP4H1, CrP4H-1, DmP4H, egg-laying abnormal-9 prolyl hydroxylase, Egl nine homolog, EGLN, EGLN prolyl hydroxylase, EGLN3, EGLN3 hydroxylase, GBAA_4459, HIF prolyl hydroxylase, HIF prolyl-4-hydroxylase, HIF-1alpha-specific prolyl-hydroxylase, HIF-P4H-1, HIF-P4H-2, HIF-P4H-3, HPH, HPH-2, HuPH4-I, HuPH4-II, hydroxylase, collagen proline, hypoxia inducible factor prolyl-4-hydroxylase domain-containing protein, hypoxia inducible factor-prolyl hydroxylase, hypoxia-inducible factor -1alpha-type prolyl 4-hydroxylase, hypoxia-inducible factor prolyl hydroxylase, hypoxia-inducible factor-1alpha-prolyl-hydroxylase 2, More, NtP4H1.1, P4H, P4H alpha1, P4H-1, P4H1, P4ha1, P4ha2, P4Halpha(I), P4Halpha(II), P4Halpha(III), P4Halpha1, PBCV-1 P4H, peptidyl proline hydroxylase, PH, PHD, PHD-1, PHD1, PHD2, PHD3, procollagen prolyl 4-hydroxylase, procollagen-proline dioxygenase, proline 4-hydroxylase, proline hydroxylase, proline protocollagen hydroxylase, proline, 2-oxoglutarate dioxygenase, proline,2-oxoglutarate 4-dioxygenase, prolyl 4-hydroxylase, prolyl 4-hydroxylase A085R, prolyl hydroxylase, prolyl hydroxylase domain 1, prolyl hydroxylase domain containing protein, prolyl hydroxylase domain enzyme, prolyl hydroxylase domain protein 2, prolyl hydroxylase-1, prolyl hydroxylase-3, prolyl-4-hydroxylase, prolyl-4-hydroxylase alpha I, prolyl-4-hydroxylase alpha II, prolyl-4-hydroxylase alpha subunit 2, prolyl-4-hydroxylase alpha1, prolyl-4-hydroxylase-alpha1, prolyl-4-hydroxylases, prolyl-glycyl-peptide, 2-oxoglutarate:oxygen oxidoreductase, 4-hydroxylating, prolyl4-hydroxylase, prolylprotocollagen dioxygenase, prolylprotocollagen hydroxylase, protocollagen hydroxylase, protocollagen proline 4-hydroxylase, protocollagen proline dioxygenase, protocollagen proline hydroxylase, protocollagen prolyl hydroxylase, PSB-II, Skp1 prolyl hydroxylase, type I C-P4H, type I proly 4-hydroxylase, type I proyl 4-hydroxylase, type II proyl 4-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.2 procollagen-proline 4-dioxygenase

Purification

Purification on EC 1.14.11.2 - procollagen-proline 4-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose fast flow anion exchange chromatography, phenyl-Sepharose fast flow column chromatography and Source 15Q anion exchange chromatography
isolation of subunits
-
isolation of subunits by ion exchange chromatography on DEAE-cellulose in 8 M urea
-
L-proline affinity chromatography and HiTrap Q Sepharose column chromatography
-
Ni-NTA agarose gel filtration
-
Ni-NTA column chromatography and gel filtration
-
Ni2+-affinity chromatography and HiLoad Superdex200 gel filtration
-
of the beta-subunit using ammonium sulfate fractionation, chromatography on preimmune IgG column and immunoadsorbent column, and gel filtration
-
of the recombinant enzyme, using an affinity purification procedure based on the use of a histidine tag in the N terminus of the protein disulfide-isomerase/beta polypeptide
of the recombinant enzyme, using anion exchange chromatography on a DEAE-cellulose column and two gel filtration steps
-
partial, using ammonium sulfate precipitation and calcium phosphate gel ion exchange
-
partial, using ammonium sulfate precipitation, column chromatography on DEAE-Sephadex A-50 and affinity chromatography on agarose-linked poly(L-proline) column
-
partial, using ion-exchange chromatography on DEAE-cellulose and gel filtration
-
partial, using ion-exchange chromatography on DEAE-cellulose, affinity chromatography with poly-L-proline coupled to tresyl-activated Sepharose-4B and gel filtration
-
recombinant C-P4H alpha(I) subunit
-
recombinant enzyme from by a process involving hydrophobic interaction chromatography purification step prior to gel filtration
Q81LZ8
recombinant enzyme from Escherichia coli strain BL21 star DE3 pLysS cells
Q81LZ8
recombinant enzyme with a N-terminal His6-SUMO fusion protein from Escherichia coli to homogeneity, the tag is cleaved off
-
recombinant isozymes
-
recombinant P4H, poly(proline)-affinity column, anion-exchange, gel filtration
-
recombinant selenomethionine-labeled anthrax-P4H from Escherichia coli by anion exchange chromatography and gel filtration toover 99% purity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Origami DE3
-
separation of 4-hydroxylase activity from 3-hydroxylase activity using affinity chromatography and gel filtration
-
separation of the enzyme from the inactive precursor of the enzyme, using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50 and Agarose A-1.5m columns
-
using affinity chromatography and column chromatography on DEAE-cellulose
-
using affinity chromatography on a column containing a polypeptide substrate of the enzyme linked to agarose, elution of the enzyme with a second peptide substrate and separation of the enzyme from this peptide by gel filtration
using affinity chromatography on a column containing poly-(L-proline) linked to agarose, elution with the same polypeptide of a lower molecular weight, and gel filtration
using affinity chromatography, ion-exchange chromatography and gel filtration
using ammonium sulfate fractionation and affinity chromatography
-
using ammonium sulfate fractionation, affinity chromatography on poly(L-proline) coupled to Sepharose 4B, DEAE-cellulose chromatography, and gel filtration
using ammonium sulfate fractionation, affinity chromatography on poly-L-proline coupled to Sepharose 4B and DEAE-cellulose chromatography
-
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and calcium phosphate gel fractionation
-
using ammonium sulfate fractionation, fractionation with calcium phosphate gel, fractionation with alumina gel, chromatography on DEAE-Sephadex and polyacrylamide gel filtration
-
using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50
-
using ammonium sulfate precipitation, affinity chromatography on poly(L-proline) column and gel filtration
-
using ammonium sulfate precipitation, calcium phosphate batch fractionations and anion exchange chromatography on DEAE-agarose followed by gel filtration on agarose
-
using chromatography on DEAE-cellulose column, affinity column, anion-exchange chromatography and a second chromatography on DEAE-cellulose column
using chromatography on DEAE-Sepharose Fast flow column, ammonium sulfate precipitation, chromatography on phenyl-Superose column, second ammonium sulfate precipitation and column chromatography on Superdex G75 HR
-
using column chromatography on DEAE-cellulose, gel filtration and affinity chromatography
-
using fractionating precipitation, ion-exchange chromatography and gel filtration
-
using fractionating precipitations, ion-exchange chromatographies and gel filtrations
-
using ion-exchange chromatography on DEAE-cellulose and affinity chromatography on a column of poly-L-proline-Sepharose 4B
-
using ion-exchange chromatography on DEAE-cellulose, and affinity chromatography on poly(L-hydroxyproline), 3,4-dihydroxyphenylacetate or 3,4-dihydroxyphenylpropionate linked to Sepharose
-
using Ni2+-chelate affinity chromatography
-