1.14.11.16: peptide-aspartate beta-dioxygenase

This is an abbreviated version!
For detailed information about peptide-aspartate beta-dioxygenase, go to the full flat file.

Reaction

Synonyms

AAH, asparaginyl hydroxylase factor inhibiting HIF-1, aspartate beta-hydroxylase, aspartate-beta-hydroxylase, aspartate/asparagine-beta-hydroxylase, aspartyl (asparaginyl) beta-hydroxylase, aspartyl (asparaginyl)-beta-hydroxylase, aspartyl beta-hydroxylase, aspartyl-(asparaginyl) beta-hydroxylase, aspartyl-(asparaginyl)-b-hydroxylase, aspartyl-(asparaginyl)-beta-hydroxylase, aspartyl-(asparagyl)-beta-hydroxylase, aspartyl-asparaginyl-beta-hydroxylase, aspartyl/asparaginyl beta-hydroxylase, aspartylpeptide beta-dioxygenase, ASPH, EGF beta-hydroxylase, EGFH, factor inhibiting HIF, factor inhibiting hypoxia-inducible factor, factor inhibiting hypoxia-inducible transcription factor (HIF), factor-inhibiting hypoxia-inducible factor, FIH, HAAH, HIF asparaginyl hydroxylase, human aspartyl (asparaginyl) beta-hydroxylase, hypoxia-inducible factor asparaginyl hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

                1.14.11.16 peptide-aspartate beta-dioxygenase

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Results	in table
2	Activating Compound
235	AA Sequence
7	Application
1	CAS Registry Number
18	Cloned(Commentary)
4	Crystallization (Commentary)
162	Disease/ Diagnostics
13	Expression
24	General Information
77	IC50 Value
87	Inhibitors
4	kcat/KM [mM/s]
25	KM Value [mM]
12	Localization
14	Metals/Ions
10	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
49	Organism
45	PDB ID
2	pH Optimum
1	pH Range
1	Posttranslational Modification
18	Protein Variants
14	Purification (Commentary)
1	Reaction
7	Reaction Type
49	Reference
61	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
48	Substrates and Products (Substrate)
3	Subunits
65	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
8	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.14.11.16 - peptide-aspartate beta-dioxygenase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

ankyrin repeat domain of endogenous Notch receptor L-asparagine + 2-oxoglutarate + O2

ankyrin repeat domain of endogenous Notch receptor 3-hydroxy-L-asparagine + succinate + CO2

2 entries

ankyrin repeat domain protein

?

Homo sapiens

-

a maximum of three ankyrin repeats enables ankyrin repeat domain proteins as a substrate

699589

-

-

?

first epidermal growth factor-like domain-L-aspartate + 2-oxoglutarate + O2

first epidermal growth factor-like domain-3-hydroxy-L-aspartate + succinate + CO2

Bos taurus

-

-

439256, 439258

-

-

r

HIF (L-Asn803) + 2-oxoglutarate + O2

HIF (3-hydroxy-L-Asn803) + succinate + CO2

Homo sapiens

-

accepts HIF-1alpha and HIF-2-alpha as substrate, HIF mutant V802A exhibits 4fold lower substrate activity than native protein, no activity with N803A mutant

659316

-

-

?

HIF-1alpha peptide Asp788-Leu822 (L-Asn803) + 2-oxoglutarate + O2

HIF-1alpha peptide Asp788-Leu822 (3-hydroxy-L-Asn803) + succinate + CO2

Homo sapiens

Q9NWT6

much lower activity with peptides lacking residues 819-822, 807-822, and 815-822

659445

-

-

?

HIF-1alpha peptide Asp788-Leu822 (L-asparagine803) + 2-oxoglutarate + O2

?

Homo sapiens

Q9NWT6

-

659445

-

-

r

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

8 entries

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

12 entries

peptide-L-aspartate + 2-oxoglutarate + O2

peptide-3-hydroxy-L-aspartate + succinate + CO2

5 entries

[factor X first EGF-like domain]-L-aspartate + 2-oxoglutarate + O2

[factor X first EGF-like domain]-3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

Q12797

-

765330

-

-

?

[thioether-linked cyclic peptide hFX-CP101-119]-L-aspartate + 2-oxoglutarate + O2

[thioether-linked cyclic peptide hFX-CP101-119]-3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

Q12797

the enzyme accepts substrates with a noncanonical EGFD disulfide connectivity (i.e. the Cys 1-2, 3-4, 5-6 disulfide pattern). Stable cyclic thioether analogues of the noncanonical EGFD AspH substrates are developed to avoid disulfide shuffling

765071

-

-

?

additional information

?

-

3 entries

ankyrin repeat domain of endogenous Notch receptor L-asparagine + 2-oxoglutarate + O2

ankyrin repeat domain of endogenous Notch receptor 3-hydroxy-L-asparagine + succinate + CO2

Homo sapiens

-

hydroxylation of highly conserved asparaginyl residues within the ankyrin repeat

687592

-

-

?

ankyrin repeat domain of endogenous Notch receptor L-asparagine + 2-oxoglutarate + O2

ankyrin repeat domain of endogenous Notch receptor 3-hydroxy-L-asparagine + succinate + CO2

Mus musculus

-

hydroxylation of hyghly conserved asparaginyl residues within the ankyrin repeat

687592

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Bos taurus

-

-

439253

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Bos taurus

-

first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18

439256, 439258

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Homo sapiens

-

-

439252, 657901, 659316, 673915, 675183, 675861, 676817

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Homo sapiens

Q9NWT6

-

659445

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Homo sapiens

-

first epidermal growth factor-like domain of bovine protein S with an asparagine replacing the aspartic acid at position 18

439254

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Homo sapiens

-

enzyme catalyzes the erythro-beta-hydroxylation of asparaginyl and aspartyl residues to form the 2S, 3R-product, possible role in the Notch signalling pathway

657901

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Homo sapiens

-

hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300

657901, 659316

-

-

?

peptide L-asparagine + 2-oxoglutarate + O2

peptide 3-hydroxy-L-asparagine + succinate + CO2

Homo sapiens

Q9NWT6

hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300

659445

-

-

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Bos taurus

-

-

439253

-

439253

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Bos taurus

-

first epidermal growth factor-like domain of human protein S as substrate

439258

-

439258

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Bos taurus

-

first epidermal growth factor-like domain of human protein S as substrate

439259

-

439259

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Bos taurus

-

specific erythro-hydroxylation

439259

-

439259

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Bos taurus

-

second epidermal growth factor-like domain of bovine protein S

439255

-

439255

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

-

first epidermal growth factor-like domain of bovine protein S as substrate

439252

-

439252

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

-

hydroxylates epidermal growth factor-like domains in transformation-associated proteins

439252

-

439252

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Mus musculus

-

-

439251

-

439251

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Mus musculus

-

specific erythro-hydroxylation

439260

-

439260

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Mus musculus

-

first epidermal growth factor-like domain of human factor IX as substrate

439260

-

439260

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Rattus norvegicus

-

specific erythro-hydroxylation

439260

-

439260

?

peptide L-aspartate + 2-oxoglutarate + O2

peptide 3-hydroxy-L-aspartate + succinate + CO2

Rattus norvegicus

-

first epidermal growth factor-like domain of human factor IX as substrate

439260

-

439260

?

peptide-L-aspartate + 2-oxoglutarate + O2

peptide-3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

Q12797

-

765446, 765784

-

-

?

peptide-L-aspartate + 2-oxoglutarate + O2

peptide-3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

Q12797

the enzyme catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains

765446, 765784

-

-

?

peptide-L-aspartate + 2-oxoglutarate + O2

peptide-3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

Q12797

the enzyme catalyzes the post-translational hydroxylation of Asp and Asn residues in epidermal growth factor-like domains

765071

-

-

?

peptide-L-aspartate + 2-oxoglutarate + O2

peptide-3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

Q12797

the enzyme accepts epidermal growth factor-like domain (EGFD) substrates with a noncanonical (i. e., Cys 1-2, 3-4, 5-6) disulfide pattern. Synthetic thioether linked cyclic peptide, hFX-CP101-119 is used as substrate. hFX-CP101-119 is designed based on 19 EGFD1 amino acid residues of the sequence of human coagulation factor X (hFX amino acids 101-119)

764503

-

-

?

peptide-L-aspartate + 2-oxoglutarate + O2

peptide-3-hydroxy-L-aspartate + succinate + CO2

Homo sapiens

Q12797

the substrate is the synthetic thioether linked cyclic peptide hFX-CP101-119. It is designed based on 19 EGFD1 amino acid residues of the sequence of human coagulation factor X (hFX amino acids 101-119)

764282

-

-

?

additional information

?

-

Homo sapiens

-

overexpression may be associated with malignant transformation

439252

-

-

?

additional information

?

-

Homo sapiens

-

the enzyme hydroxylates epidermal growth factor-like domains in transformation-associated proteins

725118

-

-

?

additional information

?

-

Mus musculus

-

overexpression may be associated with malignant transformation

439251

-

-

?