1.14.11.1: gamma-butyrobetaine dioxygenase

This is an abbreviated version!
For detailed information about gamma-butyrobetaine dioxygenase, go to the full flat file.

Reaction

Synonyms

4-trimethylaminobutyric acid dioxygenase, alpha-butyrobetaine hydroxylase, BBD, BBH, BBOX, BBOX1, Bu hydroxylase, butyrobetaine hydroxylase, gamma butyrobetaine hydroxylase, gamma-BBD, gamma-BBH, gamma-buryrobetaine dioxygenase, gamma-butyrobetaine dioxygenase, gamma-butyrobetaine hydroxylase, gamma-butyrobetaine hydroxylase 1, GBB hydroxylase, GBBH, hBBOX, oxygenase, gamma-butyrobetaine di-, psBBOX

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

                1.14.11.1 gamma-butyrobetaine dioxygenase

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Results	in table
27	Activating Compound
1493	AA Sequence
7	Application
1	CAS Registry Number
8	Cloned(Commentary)
15	Cofactor
4	Crystallization (Commentary)
46	Disease/ Diagnostics
4	Expression
26	General Information
4	General Stability
80	IC50 Value
157	Inhibitors
7	Ki Value [mM]
53	KM Value [mM]
15	Localization
25	Metals/Ions
13	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
88	Organism
2	Oxidation Stability
6	Pathway
17	PDB ID
9	pH Optimum
2	pH Range
14	Purification (Commentary)
4	Reaction
4	Reaction Type
55	Reference
61	Source Tissue
20	Specific Activity [micromol/min/mg]
5	Storage Stability
91	Substrates and Products (Substrate)
18	Subunits
51	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
2	Temperature Stability [°C]
18	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.14.11.1 - gamma-butyrobetaine dioxygenase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C to 4°C, 50% loss of activity in a week

Pseudomonas sp.

-

439284

-60° to -170°C, stable for 6 months in potassium phosphate buffer, pH 6.5

Pseudomonas sp.

-

439284

-90°C, stable for a year or more as acetone powder, stable for 3 months as purified and lyophilized enzyme

Bos taurus

-

439286

4°C, 50 mM sodium phosphate pH 7.4, 20 mM KCl, 10 mM DTT, 200g/l glycerol are best conservation conditions

Rattus norvegicus

-

439300

addition of EDTA causes total loss of activity

Rattus norvegicus

-

439300