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1.14.11.1: gamma-butyrobetaine dioxygenase

This is an abbreviated version!
For detailed information about gamma-butyrobetaine dioxygenase, go to the full flat file.

Word Map on EC 1.14.11.1

Reaction

4-Trimethylammoniobutanoate
+
2-oxoglutarate
+
O2
=
3-hydroxy-4-trimethylammoniobutanoate
+
succinate
+
CO2

Synonyms

4-trimethylaminobutyric acid dioxygenase, alpha-butyrobetaine hydroxylase, BBD, BBH, BBOX, BBOX1, Bu hydroxylase, butyrobetaine hydroxylase, gamma butyrobetaine hydroxylase, gamma-BBD, gamma-BBH, gamma-buryrobetaine dioxygenase, gamma-butyrobetaine dioxygenase, gamma-butyrobetaine hydroxylase, gamma-butyrobetaine hydroxylase 1, GBB hydroxylase, GBBH, hBBOX, oxygenase, gamma-butyrobetaine di-, psBBOX

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
                1.14.11.1 gamma-butyrobetaine dioxygenase

Purification

Purification on EC 1.14.11.1 - gamma-butyrobetaine dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme by nickel affinity chromatography, gel filtration, ultrafiltration, and buffer exchange via desalting gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography, to over 90% purity
recombinant His-tagged GBBH from Escherichia coli strain WK6 by nickel affinity chromatography
recombinant His6-tagged enzyme from Saccharomyces cerevisiae strain AH22 by nickel affinity chromatography, desalting gel filtration, anion exchange chromatography, tag cleavage by recombinant TEV protease, desalting gel filtration, and another step of nickel affinity chromatography to remove the His-tag