1.14.11.1: gamma-butyrobetaine dioxygenase

This is an abbreviated version!
For detailed information about gamma-butyrobetaine dioxygenase, go to the full flat file.

Reaction

Synonyms

4-trimethylaminobutyric acid dioxygenase, alpha-butyrobetaine hydroxylase, BBD, BBH, BBOX, BBOX1, Bu hydroxylase, butyrobetaine hydroxylase, gamma butyrobetaine hydroxylase, gamma-BBD, gamma-BBH, gamma-buryrobetaine dioxygenase, gamma-butyrobetaine dioxygenase, gamma-butyrobetaine hydroxylase, gamma-butyrobetaine hydroxylase 1, GBB hydroxylase, GBBH, hBBOX, oxygenase, gamma-butyrobetaine di-, psBBOX

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

                1.14.11.1 gamma-butyrobetaine dioxygenase

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27	Activating Compound
1493	AA Sequence
7	Application
1	CAS Registry Number
8	Cloned(Commentary)
15	Cofactor
4	Crystallization (Commentary)
46	Disease/ Diagnostics
4	Expression
26	General Information
4	General Stability
80	IC50 Value
157	Inhibitors
7	Ki Value [mM]
53	KM Value [mM]
15	Localization
25	Metals/Ions
13	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
88	Organism
2	Oxidation Stability
6	Pathway
17	PDB ID
9	pH Optimum
2	pH Range
14	Purification (Commentary)
4	Reaction
4	Reaction Type
55	Reference
61	Source Tissue
20	Specific Activity [micromol/min/mg]
5	Storage Stability
91	Substrates and Products (Substrate)
18	Subunits
51	Synonyms
1	Systematic Name
21	Temperature Optimum [°C]
2	Temperature Stability [°C]
18	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.14.11.1 - gamma-butyrobetaine dioxygenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

analysis of a crystal structure of human BBOX in complex with inhibitor AR692B and Ni2+ (an Fe2+ surrogate), P212121 space group, 2.8 A resolution

Homo sapiens

O75936

744690

crystal structure analysis, PDB ID 3O2G

Homo sapiens

O75936

745953

purified recombinant enzyme in complex with inhibitors 4-(ethyldimethylammonio)butanoate, 3-carboxy-N-(2-fluoroethyl)-N,N-dimethylpropan-1-aminium, 1-(3-carboxypropyl)-1-methylpyrrolidin-1-ium, 4-(trimethylammonio)pentanoate, [3-(trimethylammonio)propyl]phosphinate, and 3-(1,1,1,2-tetramethylhydrazin-1-ium-2-yl)propanoate, sitting drop vapor technique, mixing of 0.001 ml of 7 mg/ml protein, 8 mM N-oxalylglycine and 4 mM inhibitor, with 0.001 ml of well solution containing 0.2 M ammonium citrate, 20% PEG 3350, 3% hexanediamine, and 10 mM ZnSO4, pH 7.0, X-ray diffraction structure determination and analysis at 1.70-2.40 A resolution

Homo sapiens

O75936

745521

purified recombinant His-tagged GBBH, and SeMet-labeled variant, by sitting drop vapor diffusion technique, mixing 0.001 ml of 7 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 1.2 M ammonium sulfate and 100 mM sodium acetate, pH 4.5. In co-crystallization trials with ligands, each ligand is added to the reservoir solution to a final concentration of 5 mM, X-ray diffraction structure determination and analysis at 2.0 A and 3.5 A resolution, respectively

Homo sapiens

O75936

711108